Survey of ALV Status in Beijing Suburb Chicken Population and Development of PCR for Detected ALV

doi:10.7668/hbnxb.2010.04.045

Abstract

Abstract: ELISA was used to detect the P27 antigen of avian leukosis virus (ALV-P27) in fecal swabs of two flocks in one chicken breeder farm in Beijing suburb，a nd serum samples of corresponding flock were collected for detection antibody of ALV-J and ALV-AB. The results showed that the detection rate of ALV-P27 was significant difference in flock A. The detection rate of ALV-P27 was respectively 0. 73% (2 /273) at 0 day， 45. 06% (114 /253) at 8 weeks，and the rate of ALV-P27 was 0(0 /182) at 0 day and 45. 52% (66 /145) at 14 weeks in flock B. The detection rate of antibody of ALV-J was 35. 71% (15 /42) ，27. 08% (13 /48) in flock A and 4. 00% (2 /50) ，2. 33% (1 /43) ， 9. 30% (4 /43) in flock B. The detection rate of antibody of ALV-AB was 8. 33% (4 /48) in flock A and 6. 98% (3 /43) 、6. 98% (3 /43) in flock B. Simultaneously，a mplified by PCR and sequenced. The specific fragment of 717 bp for P27 and 545bp for ALV-J was amplified from samples. The amplified 717 bp fragment shared 91. 3% and 94. 7% identities in nucleotide with that of HPRS-103 and NX0101 strains of ALV-J. And 545 bp fragment shared 97. 1%－97. 3% and 98. 1% -98. 6% identities in nucleotide with HPRS-103 and NX0101 strains of ALV-J.

Key words: Avian leukosis virus, ALV-P27, PCR

CLC Number:

S855.3

WANG Gai-li, LIU Hua-gui, CHU Qin, ZHANG Jian, ZHAO Quan, ZHANG Li. Survey of ALV Status in Beijing Suburb Chicken Population and Development of PCR for Detected ALV[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 213-217. doi: 10.7668/hbnxb.2010.04.045.

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