Study on the Genetic Stability of Transgenic Bt+Sck Insect-resistant Cotton

doi:10.7668/hbnxb.2010.03.013

Abstract

Abstract: The research on the genetic stability of transgenic plants is necessary,which is important to further use the transgenic plants.The transgenic Bt + Sck insect-resistant cotton( 312-5T₂, 332-2T₂ and their successive generations T₀,T₁,T₂,T₃,T₄ ) were studied in present paper.The insect-resistant transgenes were stably transmitted over successive generations monitored by Southern Blot analysis and RT-PCR.The leaf bioassays showed no significantly difference in the progenies of transformed plants till T₄, indicating the resistance to bollworm was stably inherited over successive generations.The transgenic Bt + Sck insect-resistant cotton would be valuable to develop new transgenic cotton varieties with high efficiency against cotton bollworm．

Key words: Genetic stability, Bt + Sck double genes, Cotton

CLC Number:

S562

GUO Jin-ying, ZHANG Tian-zhen. Study on the Genetic Stability of Transgenic Bt+Sck Insect-resistant Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(3): 55-57. doi: 10.7668/hbnxb.2010.03.013.

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http://www.hbnxb.net/EN/Y2010/V25/I3/55

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