Study on the Improvement of Immunogenicity of Myostatin with Hepatitis B Core Antigen

doi:10.7668/hbnxb.2009.05.041

Abstract

Abstract: Myostatin is a member of the TGF-βsuperfamily that functions as a negative regulator of skeletal muscle development in mammals.Targeting the myostatin pathway may be an effective strategy for increasing muscle growth.To improve immunogenicity of myostatin,the possibility of hepatitis B core antigen as molecular adjuvant was studied.First,myostatin C-domain was amplified by PCR and fused to the gene of hepatitis B core antigen at the position of N-terminal,C-terminal and internal respectively.Then three expression vector pET-30a-MSTN/HBcAg (N),pET-30a-MSTN/HBcAg(C) and pET-30a-MSTN/HBcAg(M)was constructed.The fused proteins were expressed in E.coli. and were purified by Ni²⁺ affinity chromatography.Then male Kunming white mice were immunized with single fused protein or recombinant C-domain.The indirect ELISA assay was used to detect the titer of antiserum against myostatin.The results shown:The immune effect of fused proteins was significantly better than that of recombinant C-domain(P<0.05);The immune effect of fused protein MSTN/HBcAg(M)was the best in three fused protein.These results proved that HBcAg could be used as molecular adjuvant to improve the immunogenicity of myostatin.

Key words: Myostatin, HBcAg, Fused protein, Immunogenicity

CLC Number:

S852.4+4

MA Yi, CHEN Xiao-qiang, WANG Wen-jie, LI Qing, GUAN Hong, AN Xiaorong, CHEN Yong-fu. Study on the Improvement of Immunogenicity of Myostatin with Hepatitis B Core Antigen[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(5): 197-200. doi: 10.7668/hbnxb.2009.05.041.

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http://www.hbnxb.net/EN/Y2009/V24/I5/197

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