A Key Limited Factor ATP of Constructing C4-like Rice

doi:10.7668/hbnxb.2009.04.004

Abstract

Abstract: With untransformed rice and transgenic rices with different C4 photosynthesis genes as the materials, the activity of C4 photosynthesis enzymes, the net photosynthetic rate ( Pn) and the indexes of act ive oxygen metabolism were determined.The activities of C4 photosynthesis enzymes in untransformed rice were very low, while those of corresponding enzymes in different transgenic rices were all highly expressed. The Pn of the PPDK transgenic rice was not increased, that of the ME transgenic rice was decreased by 6. 1%, that of the transgenic ricewith PEPC and PPDK genes( CK) was simi?? lar with that of the PEPC transgenic rice under high light intensity. After treatment with ATP and its activator NaHSO3, the photosynthetic rate of O2 evolution were significant ly increased in the CK transgenic rice and the transgenic rice with PEPC, PPDK andME genes( CKM), which reached 80. 7% of that in maize and exhibited photosynthetic character of C4?? like plants.This indicated that ATP is the key limited factor for construct ing C4??like rice. In addition, after MV( artificial photooxidative activator) treatment, the CKM transgenic ricewas more tolerant to photooxidative stress.These results would provide a new technical approach to constructing C4??like rice.

Key words: Transgenic rice, Photosynthetic characteristics, C4 photosynthetic cycle, Adenosine triphosphate(ATP)

CLC Number:

ZHANG Bian-jiang, LING Li-li, CHEN Quan-zhan, HUA Chun, JIAO De-mao. A Key Limited Factor ATP of Constructing C4-like Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 17-22. doi: 10.7668/hbnxb.2009.04.004.

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http://www.hbnxb.net/EN/Y2009/V24/I4/17

References

[1] Ku M S B,Sakae A,Mika N,et al.High level expression of maize phosphoenopyruvate carboxylase in transgenic rice plants[J].Nat Biotechnol9997:76-80.
[2] Fukayama H,Tsuchida H,Agarie S,et al.Significant accumulation of C4-specific pyruvate,orthophosphate dikinase in a C3 plant rice[J].Plant Physio,200127:1136-1146.
[3] Tsuchida H,Tamai T,Fukayama H,et al.High level expression of C4-specific NADP-malic enzyme in leaves and impairment of photoautotrophic growth in a C3 plant,rice[J].Plant Cell Physiol,2001,42:138-145.
[4] Jiao D M,Li X,Huang X Q,et al.The characteristics of CO2 assimilation of photosynthesis and chlorophyll fluorescence in transgenic PEPC rice[J].Chin Sci Bull,2001,46:414-418.
[5] Chi W,Jiao D M,Li X,et al.Photosynthetic characteristics of transgenic rice plants overexpressing maize phosphoenopyruvate carboxylase[J].Acta Bot Sin,2001,43:657-660.
[6] 黄雪清,焦德茂.转C4光合酶基因水稻株系的抗光氧化特性[J].植物生理学报,2001,27(5):393-400.
[7] Jiao D M,Li X,Ji B H.Photoprotective effects of high level expression of C4 phosphoenolpyruvate carboxylase in transgenic rice during photoinhibition[J].Photosynthetica,2005,43:501-508.
[8] Jiao D M,Kuang T Y,Li X,et al.Physiological characteristics of theprimitive CO2 concentrating mechanism in PEPC transgenicrice[J].Science in China,2003,46:438-446.
[9] Ji B H,Zhu S Q,Jiao D M.A limited photosynthetic C4-microcycle and its physiological function in transgenic rice plant expressing the Maize PEPC gene[J].Acta Bot Sin,2004,46(5):542-551.
[10] Jiao D M,Hang X Q,Li X,et al.Photosynthetic characteristics and tolerance to photooxidation of transgenic rice expressing C4 photosynthesis enzymes[J].Photosynth Res,2002,72:85-93.
[11] Ji B H,Tan H H,Zhou R,et al.Promotive effect of low concentrations of NaHSO3 on photophosphorylation and photosynthesis in phosphoenolopyruvate carboxylase transgenic rice leaves[J].Acta Bot Sin,2005,47(2):178-186.
[12] Chi W,Zhou J S,Zhang F,et al.Photosynthetic features of transgenic rice expressing sorghum C4 type NADP-ME[J].Acta Bot Sin,2004,46(7):873-882.
[13] Gonzalez D H,Iglesias A A,Andeo C S.On the regulation of phosphoenolpyruvate carboxylase activity from maize leaves by L-malate:effect of pH[J].Plant Physiol98416:425-429.
[14] Hatch M D,Sack C R.Pyruvate,Pi dikinase from leaves[J].Meth Enzymol975,42:212-219.
[15] 陈景治,陈冬兰,吴敏贤.高粱和小麦叶片苹果酸酶某些特性比较[J].植物生理学报981,7(4):345-350.
[16] 李斌,陈冬兰,施教耐.高粱NADP苹果酸脱氢酶的纯化及其分子特性[J].植物生理学报9943(2):113-121.
[17] 陈甲瑞,梁银丽,周茂娟,等.免耕和施肥对玉米光合速率的影响[J].水土保持研究,20063(6):72-74.
[18] Giannopolitis C N,Ries S K.Superoxide dismutase:I.Occurrence in higher plants[J].Plant Physiol977,59:309-314.
[19] Kochba J,Lave E S,Spiegel-Roy P.Difference in peroxidase activity and isoenzymes in embryogenic and non-embryogenic shamouti orange ovular callus lines[J].Plant Cell Physiol9778:463-467.
[20] 王爱国,罗广华.植物超氧物自由基与羟胺反应的定量关系[J].植物生理学通讯990,6:55-57.
[21] Heath R L,Packer L.Photoperoxidation in isolated chloroplast.1.Kinetics and stoichiometry of fatty acid peroidation[J].Arch Biochem Biophys96825:189-198.
[22] Dennis N.Agricultural research:Consortium aims to supercharge rice photosynthesis[J].Science,2006,28(313):423.
[23] Freitag H,Stichler W.A remarkable new leaf type with unusual photosynthetic tissue in a central Asiatic genus of Chenopodiaceae[J].Plant Biol,2000,2:154-160.
[24] Huang X Q,Jiao D M,Chi W,et al.Characteristics of CO2 exchange and chlorophyll fluorescence of transgenic rice with C4 genes[J].Acta Bot Sin,2002,44(4):405-412.
[25] 崔继林.光合作用与生产力[M].南京:江苏科学技术出版社,2000:347.
[26] Mikio N,Fang Q,Lisa A B,et al.Laser-capture microdissection,a tool for the global analysis of gene expression in specific plant cell types:identification of genes expressed differentially in epidermal cells or vascular tissues of maize[J].The Plant Cell,20035:583-596.

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