Degradation of Non-target Genes During Feed Processes of Genetically Modified Soybean Meal

doi:10.7668/hbnxb.2009.02.041

Abstract

Abstract: In order to privide some knowledge for transgenic plant feed, primeres were designed on transgenic soy2 bean meal within CaMV35 S promoter gene and NOS terminator, and effect of heat treatment, autoclave, and other process2 ing technics on degradation of CaMV35 S and NOS in soybean meal was investigated by polymerase chain reaction detec2 tion system in this experiment.The results showed that heat treatment had no effect on degradation of both CaMV35S and NOS.Autoclave treatment also did not affect degradation of CaMV35S,and 246,165,and 101 bp fragments of CaMV35S were all detectable,but resulted in degradation of NOS,and 125 bp fragment was detectable and 217 bp fragment was undetectable.And the same result was obtained also in feeds those processed fromsoybean meal.The promoter gene was relatively stable and terminator gene was relatively easily destroyed.

Key words: Soybean meal, Promoter, Terminator, Feed processing

CLC Number:

LIANG Shan, LIANG Ning, JIANG Ji-zhi1, HU Dong-sheng. Degradation of Non-target Genes During Feed Processes of Genetically Modified Soybean Meal[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(2): 201-205. doi: 10.7668/hbnxb.2009.02.041.

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