Construction of the cDNA Library from IJs of Steinernema feltiae G26 and Analysis of Expressed Sequence Tags

doi:10.7668/hbnxb.2008.06.016

Abstract

Abstract: Entomopathogenic nematodes(EPN)is a new type of bio-pesticide which has great potential for controlling many important agricultural pests.The infective juveniles(IJ s)play an important role in its commercialization.A full length cDNA library from IJ s of Steinernema feltiae G26 was constructed using SMART techniques.The titer of primary cDNA library was 3.7×10⁸cfu/mL.Sequencing and sequence similarity searches were performed to analyze theprofile of expressed sequence tags(ESTs).386 qualified sequences were obtained from 450 clones,the insert size ranged from 0.3-3.0 kb.ESTs sequences similarity was compared in non-redundancies database of GenBank.The results showed that 4.08 % ESTs had none homology and these which had high homology included ribosomal protein,translation elongation factor,signal transduction gene,metabolize enzyme and so on.Therefore,a cDNA library ofSteinernema feltiaeG26 has been successfully constructed.It provides basic information for understanding the mechanisms on formation,development and persistence of IJ s.

Key words: Entomopathogenic nematodes, SMART techniques, cDNA library

CLC Number:

S432.4+

MA Juan, ZHANG Jun-ge, LI Xiu-hua, LI Min-quan, CHEN Shu-long. Construction of the cDNA Library from IJs of Steinernema feltiae G26 and Analysis of Expressed Sequence Tags[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 72-76. doi: 10.7668/hbnxb.2008.06.016.

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