Identification of the Transformation-competent Artificial Chromosome(TAC)Clones Specific to the Linkage Group of Chromosomes Containing Stress-tolerant Genes ofLeymus multicaulis

doi:10.7668/hbnxb.2008.05.002

Abstract

Abstract: Leymus multicaulis(2n=4X=28,XmXmNsNs)is one of benefit native grass species in Xinjiang,which has many excellent traits such as immune or highly resistant to BYDV(Barley yellow dwarf virus),and highly tolerant to salt,drought,leanness,pollution,aphids and so on.So it is very important to isolate and clone genes controlling these traits inLeymus multicaulis.In this research,the transformation-competent artificial chromosome(TAC)genomic libraries ofLeymus multicauliswere screened through the method of colony blot hybridization in two steps with the probes of specific SSR molecular markers from stress-tolerance chromosomes,which contained stress-tolerant genes of Leymus multi-caulis respectively added to wheat alien disomic addition line.Line 24,tolerant to BYDV and aphids;Line 15,tolerant to salt and drought,and wheat alien disomic translocation-addition line;Line 14,tolerant to salt.As a result,a total of 77 positive clones respectively specific to six SSR markers from three stress tolerance chromosome linkage groups were identified.The accuracy of the screening results was verified visually by physical localization of two TAC clones 15J18 and 09I02 from the positive clones specific to a SSR marker of the same linkage group using double-color fluorescent in situ hybridization(Double-color FISH).The research results will play a pivotal role in identifying individual stress tolerance chromosome ofLeymus multicaulisand constructing high-resolution physical map.

Key words: Leymus multicaulis, TAC clones, Disomic alien(translocation)addition line, Stress-tolerance, Double- color fluorescent in situ hybridization

CLC Number:

ZHANG Yan, ZHAO Mao-lin, SUN Miao, XU Yue-yu. Identification of the Transformation-competent Artificial Chromosome(TAC)Clones Specific to the Linkage Group of Chromosomes Containing Stress-tolerant Genes ofLeymus multicaulis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(5): 6-12. doi: 10.7668/hbnxb.2008.05.002.

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