Cloning and Sequence of ETR1 Gene from Citrullus lanatus and Cucumis sativus

doi:10.7668/hbnxb.2009.05.013

Abstract

Abstract: ETR1 was the controlling gene in ethylene signal transduction.A pair of oligo nucleotide primers were designed from conserved domain of ETR1 gene family.PCR amplifications were performed on genomic DNA template of Citrullus lanatus (Thunb.) Matsum &Nadai var.lanatus and Cucumis sativus L.,they produced two fragments of 1633 bp and 1491 bp,named CLETR1 and CSETR1 respectively.The results of Blastn on NCBI Genebank database indicated that many highly matched homologous nucleic acid sequences and amino acid sequences were all ethylene receptor gene,the ratio were 80%-95% and 75%-90% respectively.The single nucleotide variations were found in the conserved sequences between CLETR1 and CSETR1,there were 23 SNPs (single nucleotide polymorphisms) in the encoding region,and 5 of them resulted 4 amino acids difference.

Key words: ETR1, Gene cloning,Citrullus lanatus(Thunb.) Matsum &Nadai var.lanatus, Cucumis sativusL.

CLC Number:

S651
Q781

CAO Di, XU Yong, GUO Shao-gui, ZHAO Yue, GONG Guo-yi, ZHANG Hai-ying. Cloning and Sequence of ETR1 Gene from Citrullus lanatus and Cucumis sativus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(5): 59-63. doi: 10.7668/hbnxb.2009.05.013.

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