Analysis of Rice Cytochrome P450 Gene Differential Expression under NaCl Treatment Induced by Elicitor

doi:10.7668/hbnxb.2008.01.006

Abstract

Abstract: Cytochrome P450 genes showed differential expression in R6 seedlings′ root cells induced by elicitor then1 %NaCl treatment using Oligo DNA microarray,1 %NaCl treatment as the control;differentially expressed cytochromeP450 genes were detected by semi2quantitative RT2PCR in R6 seedlings′root cells induced by elicitor then 1 %NaCltreatment,1 %NaCl treatment and H₂O as the controls. The result showed that P450 gene OsHI21 had special expressionunder 1 %NaCl treatment and strengthened expression when induced by elicitor2HI.

Key words: Rice, Oligo DNA microarray, Elicitor, Cytochrome P450, RT2PCR

CLC Number:

S51112

CHEN Xiao, FANG Liang-jun, XIE Tian, LIANG Qi-biao. Analysis of Rice Cytochrome P450 Gene Differential Expression under NaCl Treatment Induced by Elicitor[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(1): 28-31. doi: 10.7668/hbnxb.2008.01.006.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2008.01.006

http://www.hbnxb.net/EN/Y2008/V23/I1/28

References

[1] 纪凤高,邓景扬.秋水仙素与二甲基亚砜混合水溶液处理小麦 × 黑麦杂交一代苗进行染色体加倍的试验[J].遗传,1987,9(3):1-4.
[2] Novak FJ. Production of garlic(Alium sativum L.)tetraploidsin shoottip in vitro euhure[J]. Z Pflanzenzuchtg,1983,91:329-333.
[3] 王青秀,吴纯启,廖明阳.细胞色素 P450 表达的诱导机制及其筛选方法的研究进展[J]. 国外医学药学分册,2003,30(1):43-46.
[4] 陈秋霞,黄炯烈. 昆虫细胞色素 P450 的诱导和抗药性
[J].热带医学杂志,2001,1(1):67-71.
[5] Kawasaki S,Borchert C,Devhokos M, et al. Gene expressionprofile during the initial phase of salt stress in rice[J]. PlantCell,2001,13:889-905.
[6] 方良俊,杨科伟,简　倩. 诱抗剂对水稻耐盐性的特效诱导试验[J].广东农业科学,2006(4):10-13.
[7] Ueda A,Shi W,Nakamura T,et al. Analysis of salt2induciblegenes in barley roots by differential display[J]. Journal ofPlant Research,2002,115(1118):119-130.
[8] 萨姆布鲁克J,弗里奇 E. F,曼尼阿蒂斯 T. 分子克隆实验指南[M].上海:科学出版社,1992.
[9] Lemieux B,Aharoni A,Schena M. Overview of DNA chiptechnology[J].Molecular Breeding,1998,4:277-289.
[10] Le Bouquin R,Pinot F,Benveniste,et al. Cloning and func2tional characterization of CYP94A2, a medium chain fattyacid hydroxylase from Vicia sativa[J].Biochem Biophys ResCommun,1999,261:156-162.
[11] Tijet N,Helvig C,Pinot F, et al. Functional expression inyeast and characterization of a clofibrate2inducible plant cy2tochrome P2450(CYP94A1)involved in cutin monomers syn2thesis[J].BiochemJ,1998,332:583-589.
[12] Bozak K R, Yu H,Sirevag R, et al. Sequence analysis ofripening2related cytochrome P2450 cDNAs from avocadofruit[J]. Proc Natl Acad Sci USA,1999,87:3904-3908.
[13] Frey M, K liem R,Saedler H, et al. Expression of a cy2tochrome P450 gene family in maize[J]. Mol Gen Genet,1995,246:100-109.
[14] Frey M,Chomet P,G lawischnig E,et al.Analysisof a chem2ical plant defense mechanism in grasses[J]. Science,1997,277:696-699.
[15] 冷欣夫,邱星辉. 细胞色素 P450 酶系的结构、功能与应用前景[M].北京:科学出版社,2001.
[16] Irmler S, Schroeder G, StPierre B, et al. Indole alkaloidbiosynthesis in Catharanthus roseus:new enzyme activitiesand identification of cytochrome P450 CYP72A1 as secolo2ganin synthase[J]. Plant J,2000,24:797-804.
[17] Lopes Cardoso M I,Meijer A H,Rueb S, et al. A promoterregion that controls basal and elicitor2inducible expressionlevels of the NADPH:cytochrome P450 reductase gene(Cpr)from Catharanthus roseusbinds nuclear factor GT21[J]. MolGen Genet,1997,256:674-681.
[18] Mangold U,Schroeder G,Marner FJ,et al.Molecular analy2sis and heterologous expression of an inducible cytochromeP2450 proteinfromperiwinkle(Catharanthus roseus L.)[J].Plant Physiol,1992,100:998-1007.
[19] 刘德立,张　山,赵　莉. 细胞色素 P450 与农药相互作用及其机理研究[J].华中师范大学学报(自然科学版),2005,39(4):518-524.
[20] Liang2Jun Fang,Xiao2Qin Fu,Qun2Shan Ye,et al. Effects ofP2glycoprotein inhibitor and elicitor on the salt tolerance ofrice seedlings[J].Journal of Integrative Plant Biology,2007,49(5):1-5.
[21] 刘　宛,李培军,周启星,等. 植物细胞色素 P450 酶系的研究进展及其与外来物质的关系[J]. 环境污染治理技术与设备,2001,2(5):1-9.
[22] 钟　兰,王　凯,谭　军,等. 水稻细胞色素 P450 超基因家族成员及其 EST证据[J].中国科学(C辑),2002,32(6):500-504.

Please choose a citation manager

Content to export