Abstract: For quickly establishing the virus-free lines of Ziziphus jujube Mill cv.′Langjiayuan′,the different explants and surface sterile methods,the effects of different media on the proliferation and the rooting were studied.The results showed that the explants from the water-culturing one-year young shoot tips in dormancy and the surface-sterilization with hydrargyrum solution for 5-6 min showed the best results,which contaminating rate was only 5%.During the 30 d subculture,the plantlets proliferated the best on the medium of MS+ AC 0.3 g/L+BA 1.5+KT 0.5+CM 50 mL/L.The plantlet growed(4.30±0.46)cm height and strong with normal leaf color.The rooting medium of 0.5 MS +IBA 1.5 performed good efficiency on callus-inducing,rooting and root length.The diameter of the calli reached to(0.45±0.04) cm and the root number was more than(5.0±0.82) after 3-week culture.The length of the new roots was(4.4±0.33) cm after 6-week culture.Therefore,the explants from the 1-year′s shoot tips in dormancy after water-culture,the surface sterile method with hydrargyrum for 56 min were proved as the best choice.The better proliferation medium was MS+ AC 0.3 g/L supplemented with BA 1.5,KT 0.5 and CM 50 mL/L for the sub-culture and the rooting medium was 0.5 MS +IBA 1.5 in Ziziphus jujube Mill cv.′Langjiayuan′ respectively.

Key words: Ziziphus jujube Mill cv .′Langjiayuan′, Tissue culture, Proliferation, Rooting, Culture medium, Explant

CLC Number: 

• S665.1