Abstract: The SSR amplified results of soybean was studied to optimize several factors applied in SSR technique system, including the concentration of template DNA, dNTP, primer and Taq polymerase. Template DNA and dNTP were found to have distinct effect to amplification in the range of experimentation, primer has a little effect between 0.05 μmol/L and 0.2 μmol/L, concentration of Taq polymerase have some effect to amplification, annealing temperature specific to primer pair was also checked. Optimized SSR system for soybean was established: 20 ng of template DNA, 200 μmol/L dNTP, 0.15 μmol/L of each primer, 0.5 U Taq polymerase and 1.5 mmol/L 10×Taq Buffer were added to the 20 μL PCR mixture.

Key words: Soybean, SSR, Reaction system

CLC Number: 

• S565.101