Construction of VP1 Protein and Prediction of B Cell Epitopes in VP1 from a Foot-and-mouth Disease Virus Strain OA/58

doi:10.7668/hbnxb.2007.04.041

Abstract

Abstract: Foot-and-mouth disease virus strain OA/58 RNAs were used as templates for RTPCR. The amplified cDNA products were cloned into pGEM-T easy vectors and transformed into JM109. The recombinant plasmids were identified by electrophoresis, PCR, and EcoR I cleavage. By means of homology modeling the FMDV strain OA/58 VP1 protein, the 3D mold was obtained. In order to find out B cell epitopes of OA/58 VP1, several methods were analyzed including physical and chemical characters, hydrophilicity, antigenicity. Many distinct antigenic epitopes in FMDV OA/58 VP1 were identified by computation: 2-11, 15-35, 38-50, 77-88, 90-107, 121-125, 131-135, 140-149, 154-163, 169-175, 178-189, 197-213. Application of homology molding OA/58 VP1 to predict B cell epitopes is reasonable. This method offers reasonable information for researching function of OA/58 VP1, constructing its variant body and selecting new expression forms of OA/58 VP1.

Key words: Foot-and-mouth disease virus, VP1 protein, B cell epitope

CLC Number:

S852.65

ZHOU Jian-hua, CONG Guo-zheng, GAO Shan-dian, CHANG Hui-yun. Construction of VP1 Protein and Prediction of B Cell Epitopes in VP1 from a Foot-and-mouth Disease Virus Strain OA/58[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 176-179. doi: 10.7668/hbnxb.2007.04.041.

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