Abstract: To solve the problems of the complicated, expensive detection of poisonous chemical reagents of Lipoxygenases, a method examining the Lipoxygenases economically and efficiently was described in this paper. The optimal experimental conditions for the method were surveyed by comparing different concentrations of the material and electrophoresis condition of native-polyacrylamid gel electrophoresis (native PAGE) and ananalyzing soybean lipoxygenase with Native PAGE, with the near-isogenic lines of soybean lipoxygenase and a common soybean variety Jidou No. 12 as the material in the study. We find that these three lipoxygenases could be separated clearly in the condition that 0.3 mL extract in 1 mL electrophoresismaterial, the concentration of native polyacrylamide gel is 11%, the total electrophoresis time is 6 hours; the result could be achieved repeatedly and stably in the conditions mentioned above by the analysis of the move ratio of three lipoxygenases' enzymatic bands; through comparing the different null lipoxygenase bands with the natural lipoxygenase bands, as well as the measure of molecular weight, both validated the 3 lipoxygenase enzymatic bands. It is a simple and economical method that can be used as useful tools for analyzing the resources, the cultivar breeding, and the identification of the soybean Lox-free type in the process of production, machining, purchasing and so on.

Key words: Soybean, Appraisal of lipoxygenase, Native PAGE, Move ratio

CLC Number: 

• S565.101