The Effect of Ion Implantation on the Activity of Three Kinds of Protective Enzymes in 3rd-leaf Period of Wheat

doi:10.3321/j.issn:1000-7091.2007.01.004

Abstract

Abstract: Embryos of wheat varieties Jian 54, Yunong 118 and Yumai 18 were implanted by different dose rate and fluence at the energy of 30 keV. The activities of POD, CAT and SOD enzymes were determined in 3-leaf period of wheat. The result showed that CAT and POD enzyme activities after implantation were stronger than that of control; For SOD, the activity was stronger than the control in low dose, but was lower when dose exceeded 5.6×10¹⁷N⁺/cm². With the increase of dose, the enzyme activity is up, and then down. The enzyme activity were significantly different between dose rates, and the sensitivity of varieties to ion implantation were also different.

Key words: Ion implantation, Dose rate, Dosage, POD, CAT, SOD

CLC Number:

S512.01

WANG Wei-dong, ZHU Xiao-rui, SU Ming-jie, QIN Guang-yong. The Effect of Ion Implantation on the Activity of Three Kinds of Protective Enzymes in 3rd-leaf Period of Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(1): 13-16. doi: 10.3321/j.issn:1000-7091.2007.01.004.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3321/j.issn:1000-7091.2007.01.004

http://www.hbnxb.net/EN/Y2007/V22/I1/13

References

[1] 莫简. 医用自由基生物学导论[M]. 北京: 人民卫生出版社, 1989: 49-50.
[2] 吴跃进, 吴敬德, 刘贵富, 等. 离子注入水稻种子萌发过程中的自由基和SOD酶研究[J]. 作物学报, 1996, 22(3): 320-324.
[3] 邓建国, 余增亮. 氮离子束辐射种子的自由基研究[J]. 安徽农业大学学报, 1991, 18(4): 263-268.
[4] 李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
[5] 张龙翔. 生化实验方法和技术[M]. 北京: 高等教育出版社, 1997.
[6] 邹琦. 植物生理学实验指导[M]. 北京: 中国农业出版社, 2000.
[7] 汤章城. 现代植物生理学实验指南[M]. 北京: 科学出版社, 1999.
[8] 宋道军, 李红, 余增亮. N+离子注入对不同辐射敏感性微生物超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性的影响[J]. 生物物理学报, 1998, 14(2): 325-330.
[9] 王燕, 王卫东, 秦广雍, 等. 氮离子注入对拟南芥的生理影响初步研究[J]. 华北农学报, 2004, 19(1): 82-85.
[10] 孟学平, 杨恒山, 郭宏, 等. 盐胁迫对冬小麦过氧化物酶同工酶的影响[J]. 华北农学报, 2000, 15(4): 62-67.
[11] 张义贤, 雷晓波, 阎继耀. 重金属镉、镍、汞对大麦过氧化物酶同工酶的影响[J]. 华北农学报, 2000, 15(1): 27-31.
[12] 夏寿萱. 放射生物学[M]. 北京: 军事医学科学出版社, 1998.
[13] 吴少伯. 周围组织中蛋白质及同工酶的聚丙稀酰胺凝胶盘状电泳[J]. 植物生理学通讯, 1982(4): 22-25.
[14] 王鸣刚, 罗茂春. 低能碳、氮离子对甜叶菊萌发率、生长量及过氧化物酶的影响[J]. 武汉植物学研究, 2001, 19(2): 143-148.
[15] 王卫东, 闻捷, 苏明杰, 等. 低能离子注入后小麦苗期损伤效应研究[J]. 华北农学报, 2005, 20(2): 80-83.
[16] 闻捷, 李淑平, 崔党群, 等. 离子注入对小麦苗期性状的诱变效应研究[J]. 华北农学报, 2005, 20(1): 31-34.
[17] 卫增泉, 韩光武, 马受武, 等. 低能重离子注入麦胚引起深层细胞损伤的一种可能机制[J]. 核技术, 1995, 18(2): 90-93.

Please choose a citation manager

Content to export