Acta Agriculturae Boreali-Sinica

ACTA AGRICULTURAE BOREALI-SINICA ›› 2006, Vol. 21 ›› Issue (4): 48-50. doi: 10.3321/j.issn:1000-7091.2006.04.012

Special Issue: Pear； Biotechnology；

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Comparison of Pear Genomic DNA Extraction Methods

LIU Zun-chun1, BAO Dong-e2, LIAO Ming-an3   

  1. 1. College of Landscape Architecture, Henan Institution of Sci-Tech, Xinxiang453003, China;
    2. Mathematic Department, Henan Institution of Sci-Tech, Xinxiang 453003, China;
    3. College of Forestry and Horticulture, Sichuan Agriculture University, Ya'an 625014, China
  • Received:2005-11-17 Published:2006-08-28

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Abstract: Traditional CTAB method was improved by adding Vc in this experiment.The results showed that the best quality of DNA extracted from pear genome came from the method of adding 0.1 g/g of Vc when the sample was triturated.The DNA was optimal for RAPD analysis and other molecular biology researches.The results of the experiment also indicated that tender bud was the best material,which was followed by the shoot and mature leaf.

Key words: Jinhua pear, Genomic DNA, Extraction method

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LIU Zun-chun, BAO Dong-e, LIAO Ming-an. Comparison of Pear Genomic DNA Extraction Methods[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(4): 48-50. doi: 10.3321/j.issn:1000-7091.2006.04.012.

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