Induction of Several Defense Response Enzymes in Arabidopsis Seedlings by Salicylic Acid

doi:10.3321/j.issn:1000-7091.2005.01.018

Abstract

Abstract: The influence of SA on defense enzymes ( PAL,POD, PPO and LOX) activities was studied in Arabidopsis thaliana seedlings. The result showed that the activities of PAL,POD, PPO and LOX in the leaf were increased rapidly after treatment of Arabidopsis seedlings with 10 µg/mL SA. The time to reach the highest level of PAL activity was at 12 h after SA treatment,and higher level of PAL activity could maintain from 12hto48h.POD, PPO and LOX activity could reach their peaks at 48 h after SA treatment,and higher level of POD and PPO activities could maintain within 24-96 h,The change of these physiology indexes was consistent with SA induced plant disease resistance.

Key words: Arabidopsis, Salicylic acid, Defense enzymes, Induction

CLC Number:

S567.1

ZENG Qun, CHEN Hui-qin, ZHAO Shu-qing, ZHAO Zhan-jun. Induction of Several Defense Response Enzymes in Arabidopsis Seedlings by Salicylic Acid[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(1): 75-77. doi: 10.3321/j.issn:1000-7091.2005.01.018.

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References

[1] Klessig D F, Malamy J. The salicylic acid signal in plants [J]. Plant Mol Biol, 1994, 26:1439- 1458.
[2] Malamy J, Carr J P, Klessig D F, et al. Salicylic acid:a likely endogenous signal in the resistance response of tobacco to viral infection [J]. Science, 1990, 250:1002-1004.
[3] Métraux J P, Signer H, Ryals J A, et al. Increase in salicylic acid at the onset of systemic acquired resistance in cucumber [J]. Science, 1990, 250:1004- 1006.
[4] Uknes S, Winter A, Delaney T, et al. Biological induction of systemic acquired resistance in Arabidopsis [J].Mol Plant-Microbe Interact, 1993, 6:692- 698.
[5] Yalpani N, Silverman P, Wilson T M A, et al. Salicylic acid is a systemic signal and an inducer of pathogenesisrelated proteins in virus-infected tobacco [J]. Plant Cell,1991, 3:809- 818.
[6] 中国科学院上海植物生理研究所,上海市植物生理学会.现代植物生理学实验指南[M].北京:科学出版社,1999.318-324.
[7] 薛应龙.植物生理学实验手册[M].上海:上海科学技术出版社,1985.191-192.
[8] (美)F.奥斯伯,著.精编分子生物学实验指南[M].颜子颖,王海林,译.北京:科学出版社,1998.
[9] 朱广廉,钟诲文,张爱琴.植物生理学实验[M].北京:北京大学出版社,1990.37-40.
[10] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding [J]. Anal Biochem,1976, 72:248- 259.
[11] 余叔文,汤章城.植物生理与分子生物学[M].北京:科学出版社,1999.770-783.
[12] Tsuji J, Jackson E P, Gage D A, et al. Phytoalexin accumulation in Arabidopsis thaliana during the hypersensitive reaction to Pseudomonas syringae pv syringae [J].Plant Physiol, 1992, 98:1304- 1309.
[13] Mauch-Mani B, Slusarenko A J. Production of salicylic acid precursors is a major function of phenylalanine ammonia-lyase in the resistance of Arabidopsis to Peronospora parasitica [J]. Plant Cell, 1996, 8:203-212.
[14] Uknes S, Mauch-Mani B, Moyer M, et al. Acquired resistance in Arabidopsis [J]. Plant Cell, 1992, 4:645- 656.
[15] Cao H, Bowling S A, Gordon A S, et al. Characterization of an Arabidopsis mutant that is nonresponsive to inducers of systemic acquired resistance [J]. Plant Cell,1994, 6:1583- 1592.

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