The Studies on the Characteristic and Production Condition of Antibiotic Substances from Bacillus subtils B-903 Strain

doi:10.3321/j.issn:1000-7091.1994.02.019

Abstract

Abstract: Antagonistic substances which are strong antagonistic to variou plant pathology fungi can be produced by Bacillus subtilis B-903 strain seperated from apple orchard of zhengzhou.The experiment showed that cultured this bacteria on rotary shaker for 112 hours in NYDA medium and the antifungi activity of cultural filtrate reached the highest value.The pH7 of medium was the most suitable for production of antifungi substances.The production of antifungi substances varied with medium volue in 250 ml coned flastic.The rice powder and beau cake powder was the most suitable carbon source and nitrogen source respectively.Acidifing the cultural filtrate to pH4 was the best method for its storing.The minimum inhibition density of cultural filtrate to Fasarium vasinfectum is 1/2318 (cultural filtrate/medium).

Key words: Antifungi substances, Antibiotic, Bacillus subtilis

CLC Number:

S182

Wang Wenxi, Kong Jian, Zhao Baige, Cheng Hongmei, Shen Xiaocheng, Zhang Guifen. The Studies on the Characteristic and Production Condition of Antibiotic Substances from Bacillus subtils B-903 Strain[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1994, 9(2): 98-103. doi: 10.3321/j.issn:1000-7091.1994.02.019.

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