ACTA AGRICULTURAE BOREALI-SINICA ›› 1993, Vol. 8 ›› Issue (4): 1-6. doi: 10.3321/j.issn:1000-7091.1993.04.001

Special Issue: Chinese cabbage Biotechnology High impact papers

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Influence of Genotype and Donor Plant Growth Environment on Embryo genesis from Isolated Microspores in Chinese Cabbage (Brassica campestris ssp. pekinensis)

Cao Mingqing, Li Yan, Liu Fan   

  1. Beijing Vegetable Research Centre, Beijing
  • Received:1992-01-04 Published:1993-12-28

Abstract: Of 17 Chinese cabbage (Brassica campestris ssp. pekinensis) genotypes, 16 genotypes had produced microspore-derived embryos via isolated microspore culture. The production of microspore-derived embryos varied remarkably between these genotypes. The two genotypes with the highest embryo yield, T11 and CC11, produced 35928 and 34132 embryos per 100 flower buds, respectively. The growth environment (temperature) of donor plants obviously affected microspore embryogenesis. Average embryo yield of donor plants of CC11 grown in the greenhouse at 10℃/20℃ ( night/day) was 12 991.6 embryos per 100flower buds. But that of CC11 grown in the field at 10-15 ℃/0-40℃ (night/day)was 3,831.5, or 29.5% as high as the former.

Key words: Isolated microspore culture, Embryogenesis, Genotype, Donor plant, Growth temperature, Chinese cabbage (Brassica campestris ssp. pekinensis)

Cite this article

Cao Mingqing, Li Yan, Liu Fan. Influence of Genotype and Donor Plant Growth Environment on Embryo genesis from Isolated Microspores in Chinese Cabbage (Brassica campestris ssp. pekinensis)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1993, 8(4): 1-6. doi: 10.3321/j.issn:1000-7091.1993.04.001.

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