Abstract: The rootless plantlets of anther cultural wheat with 2-3 main leaves were transplanted on the MS medium of hardening plantlets containing Bio-MET 3mg/L, NAA 0.5 mg/L, KT0.5 mg/L and 8% sucrose. After four months, the hight of plants cultured in this medium was lower and number of their tillers and roots was more than that cultured in medium without Bio-MET. The shoots per tube were planted into 1.55 hills, on the average. When the temperature declined at about 6癈 in autumn,the tube shoots were transplanted in the field covered by plastic film, then the survival rate was 90.2%. When the temperature rose to 2-3癈 in early spring, the plastic film was removed, and the number of seed-setting spikes per plant which doubled naturally was 9.1, the number of seeds per plant was 249.7 and the number of seeds per spike was 27.4. They were 3.37, 6.05, 1.86 times as much as those of the plants which doubled naturally in the greenhouse, respectively. The number of seed-setting spikes per 100 plant combining doubling naturally with double manully was 289, increased 12.9% and 91.8%, compared with those doubling naturally and doubling manully, respectively.

Key words: Winter wheat, Anther culture: Tube shoot, Seed-setting spikes