Effects of Pseudomonas rhodesiae M11 on the Growth of Maize and the Structure and Function of Rhizosphere Soil Microbial Community in Medium-low Yield Fields

doi:10.7668/hbnxb.20196691

Abstract

Abstract:

Twenty-six bacterial strains were isolated and screened from the rhizosphere soil of maize in medium-low yield fields in Gaocheng District,Shijiazhuang,Hebei Province.After evaluating their plant-growth-promoting(PGP)traits, Pseudomonas rhodesiae M11,which exhibited the best overall performance,was selected for pot experiments at three cell densities(1.0×10⁵,1.0×10⁷and 1.0×10⁹CFU/mL)to assess its effects on maize growth and on the structure and function of the rhizosphere microbiome in medium and low yield fields.The results showed that compared with CK(sterilized medium),different concentrations of M11 fermentation broth significantly promoted the growth of maize seedlings,and the treatment with 1.0×10⁷CFU/mL had the best effect,and the plant height,aboveground and root biomass of maize were significantly increased.At the same time,this treatment significantly increased the activity of urease and catalase in rhizosphere soil.Metagenomic analysis showed that inoculation with M11 increased the relative abundance of beneficial bacteria such as Sphingomicrobium,Nocardioides and Lysobacter in soil,and enhanced the topological complexity of soil microbial symbiotic network.Under the condition of poor soil in medium-low yield fields,P.rhodesiae M11 may directly promote the growth of maize by dissolving phosphorus,fixing nitrogen or secreting secondary metabolites such as indole-3-acetic acid and siderophore.It may also increase the relative abundance of beneficial microorganisms and the abundance of genes related to growth promotion such as phosphorus solubilization and nitrogen fixation by increasing the activity of growth-promoting enzymes in maize soil and improving the microbial community structure,thereby providing a favorable growth environment for maize,indirectly promoting the growth and development of maize,and providing technical support for the increase of maize yield in medium-low yield soils.

Key words: Pseudomonas rhodesiae, Medium-low yield fields, Soil enzyme activity, Metagenomic sequencing, Soil microbial community structure, Soil microbial function

CLC Number:

LI Mengkai, MA Jia, PENG Jieli, JIA Nan, WANG Xu, WANG Hao, HU Dong. Effects of Pseudomonas rhodesiae M11 on the Growth of Maize and the Structure and Function of Rhizosphere Soil Microbial Community in Medium-low Yield Fields[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(5): 152-164. doi: 10.7668/hbnxb.20196691.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20196691

http://www.hbnxb.net/EN/Y2026/V41/I5/152

Figures/Tables 16

References 52

[1]	孙磊, 王丽华, 高中超, 刘卜鸣, 佟玉欣, 张磊, 王爽, 常本超. 黑龙江省玉米低温冷害致灾机理及防御措施[J]. 黑龙江农业科学, 2019(11):148-153.doi:10.11942/j.issn1002-2767.2019.11.0148.
	Sun L, Wang L H, Gao Z C, Liu B M, Tong Y X, Zhang L, Wang S, Chang B C. Disaster mechanism and prevention measures of maize chilling damage in Heilongjiang Province[J]. Heilongjiang Agricultural Sciences, 2019(11):148-153.
[2]	Luo N, Meng Q F, Feng P Y, Qu Z R, Yu Y H, Liu D L, Müller C, Wang P. China can be self-sufficient in maize production by 2030 with optimal crop management[J]. Nature Communications, 2023, 14:2637.doi:10.1038/s41467-023-38355-2. pmid: 37149677
[3]	张丽华, 翟立超, 崔永增, 董志强, 崔英淑, 张经廷, 贾秀领. 灌水对夏玉米产量及植株理化性状的影响[J]. 华北农学报, 2023, 38(6):72-80.doi:10.7668/hbnxb.20193915.
	Zhang L H, Zhai L C, Cui Y Z, Dong Z Q, Cui Y S, Zhang J T, Jia X L. Effect of irrigation on yield and plant physicochemical characters of summer maize[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(6):72-80. doi: 10.7668/hbnxb.20193915
[4]	张琳, 张凤荣, 姜广辉, 姚慧敏. 我国中低产田改造的粮食增产潜力与食物安全保障[J]. 农业现代化研究, 2005, 26(1):22-25.doi:10.3969/j.issn.1000-0275.2005.01.006.
	Zhang L, Zhang F R, Jiang G H, Yao H M. Potential improvement of medium low yielded farmland and guarantee of food safety in China[J]. Research of Agricultural Modernization, 2005, 26(1):22-25.
[5]	曾希柏, 张佳宝, 魏朝富, 宇万太, 黄道友, 徐明岗, 徐建明. 中国低产田状况及改良策略[J]. 土壤学报, 2014, 51(4):675-682.doi:10.11766/trxb201401150029.
	Zeng X B, Zhang J B, Wei C F, Yu W T, Huang D Y, Xu M G, Xu J M. The status and reclamation strategy of low-yield fields in China[J]. Acta Pedologica Sinica, 2014, 51(4):675-682.
[6]	Park M, Kim C, Yang J, Lee H, Shin W, Kim S, Sa T M. Isolation and characterization of diazotrophic growth promoting bacteria from rhizosphere of agricultural crops of Korea[J]. Microbiological Research, 2005, 160(2):127-133.doi:10.1016/j.micres.2004.10.003. pmid: 15881829
[7]	Andersen K S, Winding A. Non-target effects of bacterial biological control agents on soil protozoa[J]. Biology and Fertility of Soils, 2004, 40(4):230-236.doi:10.1007/s00374-004-0774-y.
[8]	王慧桥, 陈为峰, 诸葛玉平, 胡国庆, 于海燕, 付乃鑫, 董元杰. 两株自生固氮菌的鉴定及其应用基础研究[J]. 华北农学报, 2018, 33(4):210-216.doi:10.7668/hbnxb.2018.04.030.
	Wang H Q, Chen W F, Zhuge Y P, Hu G Q, Yu H Y, Fu N X, Dong Y J. Identification and basic application research of two abiogenous Azotobacter[J]. Acta Agriculturae Boreali-Sinica, 2018, 33(4):210-216.
[9]	Sharma S, Kulkarni J, Jha B. Halotolerant rhizobacteria promote growth and enhance salinity tolerance in peanut[J]. Frontiers in Microbiology, 2016, 7:1600.doi:10.3389/fmicb.2016.01600. pmid: 27790198
[10]	Paul D, Lade H. Plant-growth-promoting rhizobacteria to improve crop growth in saline soils:a review[J]. Agronomy for Sustainable Development, 2014, 34(4):737-752.doi:10.1007/s13593-014-0233-6. URL
[11]	Sarkar A, Ghosh P K, Pramanik K, Mitra S, Soren T, Pandey S, Mondal M H, Maiti T K. A halotolerant Enterobacter sp.displaying ACC deaminase activity promotes rice seedling growth under salt stress[J]. Research in Microbiology, 2018, 169(1):20-32.doi:10.1016/j.resmic.2017.08.005. URL
[12]	张艳丽, 陈娟, 白新明, 孙文洁, 王宁, 冯文霞. 荧光假单胞菌5B12与杀菌剂复配对黄瓜灰霉病菌的联合毒力[J]. 农药学学报, 2025, 27(4):741-746.doi:10.16801/j.issn.1008-7303.2025.0064.
	Zhang Y L, Chen J, Bai X M, Sun W J, Wang N, Feng W X. The combined activity of Pseudomonas fluorescens 5B12 blended with fungicide against Botrytis cinerea on cucumber[J]. Chinese Journal of Pesticide Science, 2025, 27(4):741-746.
[13]	Nur A, Angelika F, Mikolaj D, Robert S, Julia P, Lukasz D, Kumar P. Biocontrol potential of Pseudomonas protegens ML15 against Botrytis cinerea causing gray mold on postharvest tomato(Solanum lycopersicum var.cerasiforme)[J]. Frontiers in Plant Science, 2023, 14:1288408.doi:10.3389/fpls.2023.1288408. URL
[14]	David B V, Chandrasehar G, Selvam P N. Chapter 10-Pseudomonas fluorescens:a plant-growth-promoting rhizobacterium(PGPR)with potential role in biocontrol of pests of crops[M]// Crop Improvement Through Microbial Biotechnology.Amsterdam:Elsevier, 2018:221-243.doi:10.1016/B978-0-444-63987-5.00010-4.
[15]	Zarei T, Moradi A, Kazemeini S A, Farajee H, Yadavi A. Improving sweet corn(Zea mays L.var saccharata)growth and yield using Pseudomonas fluorescens inoculation under varied watering regimes[J]. Agricultural Water Management, 2019, 226:105757.doi:10.1016/j.agwat.2019.105757. URL
[16]	杨华, 李江, 张维, 周正富, 燕永亮, 郭嘉, 刘相国, 郝东云, 林敏, 柯秀彬. 施氏假单胞菌在玉米根际的固氮效率和促生效果研究[J]. 中国农业科技导报, 2021, 23(4):76-84.doi:10.13304/j.nykjdb.2020.0211.
	Yang H, Li J, Zhang W, Zhou Z F, Yan Y L, Guo J, Liu X G, Hao D Y, Lin M, Ke X B. Maize growth promotion and nitrogen-fixing rates by inoculation with wild-type and ammonium-excreting mutant of Pseudomonas stutzeri[J]. Journal of Agricultural Science and Technology, 2021, 23(4):76-84.
[17]	漫静, 唐波, 邓波, 李佳欢, 何玉娟, 张佳良. 羊草根际促生菌的分离筛选及促生作用研究[J]. 草业学报, 2021, 30(1):59-71.doi:10.11686/cyxb2020321.
	Man J, Tang B, Deng B, Li J H, He Y J, Zhang J L. Isolation,screening and beneficial effects of plant growth-promoting rhizobacteria(PGPR)in the rhizosphere of Leymus chinensis[J]. Acta Prataculturae Sinica, 2021, 30(1):59-71.
[18]	张峰, 种培芳, 包新光, 王雪莹. 4株红砂根际固氮菌分离鉴定及对幼苗促生作用的研究[J]. 草业学报, 2025, 34(3):144-153.doi:10.11686/cyxb2024170.
	Zhang F, Chong P F, Bao X G, Wang X Y. Isolation and identification of four strains of Reaumuria soongorica root zone nitrogen fixing bacteria and their role in seedling growth promotion[J]. Acta Prataculturae Sinica, 2025, 34(3):144-153.
[19]	Schwyn B, Neilands J B. Universal chemical assay for the detection and determination of siderophores[J]. Analytical Biochemistry, 1987, 160(1):47-56.doi:10.1016/0003-2697(87)90612-9. pmid: 2952030
[20]	李福艳, 刘晓玉, 颜静婷, 蔡燕飞. 三株产吲哚乙酸根际促生芽孢杆菌的筛选鉴定及其促生作用[J]. 浙江农业学报, 2021, 33(5):873-884.doi:10.3969/j.issn.1004-1524.2021.05.13.
	Li F Y, Liu X Y, Yan J T, Cai Y F. Isolation and identification of three indole-3-acetic acid producing plant-growth-promoting rhizosphere Bacillus sp.and their growth-promoting effects[J]. Acta Agriculturae Zhejiangensis, 2021, 33(5):873-884.
[21]	程鑫宇, 王继莲, 麦日艳古·亚生, 李明源. 盐爪爪根际土壤产IAA菌株分离及促生特性分析[J]. 草业学报, 2024, 33(4):110-121.doi:10.11686/cyxb2023205.
	Cheng X Y, Wang J L, Mairiyangu Y, Li M Y. Isolation and growth-promoting characteristics of rhizobacteria producing indole-3-acetic acid from the rhizosphere soil of Kalidium foliatum[J]. Acta Prataculturae Sinica, 2024, 33(4):110-121.
[22]	关松荫. 土壤酶及其研究法[M]. 北京: 农业出版社, 1986.
	Guan S Y. Soil enzymes and their research methods[M]. Beijing: Agricultural Press, 1986.
[23]	Zhang Y D, Wang Y, Zhou G Y, Revillini D, Liu H Y, Wu S J, Chen N, Du B M, Xu J R, Li Q W, Guo D, Delgado-Baquerizo M, Niu D C, Fu H, Yuan X B. Soil microbial networks mediate long-term effects of nitrogen fertilization on ecosystem multiservices[J]. Journal of Ecology, 2025, 113(9):2539-2558.doi:10.1111/1365-2745.70112. URL
[24]	Skoufos G, Almodaresi F, Zakeri M, Paulson J N, Patro R, Hatzigeorgiou A G, Vlachos I S. AGAMEMNON:an accurate metagenomics and metatranscriptomics quantification analysis suite[J]. Genome Biology, 2022, 23(1):39.doi:10.1186/s13059-022-02610-4.
[25]	Goswami D, Thakker J N, Dhandhukia P C. Portraying mechanics of plant growth promoting rhizobacteria(PGPR):a review[J]. Cogent Food & Agriculture, 2016, 2(1):1127500.doi:10.1080/23311932.2015.1127500.
[26]	Li H Y, Qiu Y Z, Yao T, Ma Y C, Zhang H R, Yang X L. Effects of PGPR microbial inoculants on the growth and soil properties of Avena sativa,Medicago sativa,and Cucumis sativus seedlings[J]. Soil and Tillage Research, 2020, 199:104577.doi:10.1016/j.still.2020.104577. URL
[27]	马雪晴, 冀傲冉, 郑娇莉, 曹春霞, 龚艳, 黄大野, 王蓓蓓. 植物根际促生菌促生机制及其应用研究进展[J]. 中国农业科技导报(中英文), 2025, 27(2):13-23.doi:10.13304/j.nykjdb.2023.0654.
	Ma X Q, Ji A R, Zheng J L, Cao C X, Gong Y, Huang D Y, Wang B B. Research progress on growth-promoting mechanism and application of plant growth-promoting rhizobacteria[J]. Journal of Agricultural Science and Technology, 2025, 27(2):13-23.
[28]	Yuan J J, Zhao X, Cao X Y, Wang G T, Guo Y L, Ji X N, Hu W, Li M. Effects and mechanisms of phosphate solubilizing bacteria on enhancing phytoextraction of lead from contaminated soil by Celosia cristata L.[J]. Journal of Cleaner Production, 2022, 380:135013.doi:10.1016/j.jclepro.2022.135013. URL
[29]	De La Torre-Ruiz N, Ruiz-Valdiviezo V M, Rincón-Molina C I, Rodríguez-Mendiola M, Arias-Castro C, Gutiérrez-Miceli F A, Palomeque-Dominguez H, Rincón-Rosales R. Effect of plant growth-promoting bacteria on the growth and fructan production of Agave americana L.[J]. Brazilian Journal of Microbiology, 2016, 47(3):587-596.doi:10.1016/j.bjm.2016.04.010. pmid: 27268113
[30]	Shridhar B S. Nitrogen fixing microorganisms[J]. International Journal of Microbiological Research, 2012, 3(1): 46-52. doi:10.5829/idosi.ijmr.2012.3.1.61103.
[31]	Ni K, Pacholski A S. Soil moisture and temperature effects on granule dissolution and urease activity of urea with and without inhibitors:an incubation study[J]. Agriculture, 2022, 12(12):2037.doi:10.3390/agriculture12122037.
[32]	赵春建, 李玉正, 关佳晶, 苏伟然, 田瑶, 王婷婷, 李申, 李春英. 东北红豆杉-无花果复合种植对两种植物生长和土壤酶活性影响[J]. 植物研究, 2020, 40(5):679-685.doi:10.7525/j.issn.1673-5102.2020.05.006.
	Zhao C J, Li Y Z, Guan J J, Su W R, Tian Y, Wang T T, Li S, Li C Y. Effect of interplanting Taxus cuspidata with Ficus carica on growth of two plants and activities of soil enzymes[J]. Bulletin of Botanical Research, 2020, 40(5):679-685.
[33]	孙文颖, 马维伟, 李广, 吴江琪, 许延昭. 尕海湿地植被退化过程中土壤蔗糖酶和淀粉酶活性的动态特征[J]. 草地学报, 2019, 27(1):88-96.doi:10.11733/j.issn.1007-0435.2019.01.012.
	Sun W Y, Ma W W, Li G, Wu J Q, Xu Y Z. Dynamic characteristics of soil sucrase and amylase activities during vegetation degradation in Gahai wetland[J]. Acta Agrestia Sinica, 2019, 27(1):88-96.
[34]	张丽莉, 武志杰, 陈利军, 陈振华, 张玉兰. 不同种植制度土壤氧化还原酶活性和动力学特征[J]. 生态环境学报, 2009, 18(1):343-347.doi:10.3969/j.issn.1674-5906.2009.01.063.
	Zhang L L, Wu Z J, Chen L J, Chen Z H, Zhang Y L. Soil oxidoreductase activity and its kinetic characteristics under different cropping system[J]. Ecology and Environmental Sciences, 2009, 18(1):343-347.
[35]	闫丽娟, 王海燕, 李广, 吴江琪. 黄土丘陵区4种典型植被对土壤养分及酶活性的影响[J]. 水土保持学报, 2019, 33(5):190-196,204.doi:10.13870/j.cnki.stbcxb.2019.05.028.
	Yan L J, Wang H Y, Li G, Wu J Q. Effects of four typical vegetations on soil nutrient and enzymes activities in Loess Hilly Region[J]. Journal of Soil and Water Conservation, 2019, 33(5):190-196,204.
[36]	Cassán F, Perrig D, Sgroy V, Masciarelli O, Penna C, Luna V. Azospirillum brasilense Az39 and Bradyrhizobium japonicum E109,inoculated singly or in combination,promote seed germination and early seedling growth in corn(Zea mays L.)and soybean(Glycine max L.)[J]. European Journal of Soil Biology, 2009, 45(1):28-35.doi:10.1016/j.ejsobi.2008.08.005. URL
[37]	Díaz-Zorita M, Fernández-Canigia M V. Field performance of a liquid formulation of Azospirillum brasilense on dryland wheat productivity[J]. European Journal of Soil Biology, 2009, 45(1):3-11.doi:10.1016/j.ejsobi.2008.07.001. URL
[38]	Tang L, Shi Y M, Zhang Y L, Yang D H, Guo C H. Effects of plant-growth-promoting rhizobacteria on soil bacterial community,soil physicochemical properties,and soil enzyme activities in the rhizosphere of alfalfa under field conditions[J]. Diversity, 2023, 15(4):537.doi:10.3390/d15040537. URL
[39]	Salitha, Gopal K S.Comparative assessment of soil bacterial diversity as influenced by novel biofertilizer application in nutmeg using metagenomic analysis[J]. Biologia, 2025, 80(7):1835-1844.doi:10.1007/s11756-025-01944-1.
[40]	Shahina M, Hameed A, Lin S Y, Hsu Y H, Liu Y C, Cheng I C, Lee M R, Lai W A, Lee R J, Young C C. Sphingomicrobium astaxanthinifaciens sp.nov.,an astaxanthin-producing glycolipid-rich bacterium isolated from surface seawater and emended description of the genus Sphingomicrobium[J]. International Journal of Systematic and Evolutionary Microbiology, 2013, 63(Pt9):3415-3422.doi:10.1099/ijs.0.047704-0. URL
[41]	Qian G L, Hu B S, Jiang Y H, Liu F Q. Identification and characterization of Lysobacter enzymogenes as a biological control agent against some fungal pathogens[J]. Agricultural Sciences in China, 2009, 8(1):68-75.doi:10.1016/S1671-2927(09)60010-9. URL
[42]	Meena K K, Bitla U M, Sorty A M, Singh D P, Gupta V K, Wakchaure G C, Kumar S. Mitigation of salinity stress in wheat seedlings due to the application of phytohormone-rich culture filtrate extract of methylotrophic actinobacterium Nocardioides sp.NIMMe6[J]. Frontiers in Microbiology, 2020, 11:2091.doi:10.3389/fmicb.2020.02091. URL
[43]	王超, 陈娟, 沈仁芳. 酸性红壤上玉米不同部位固氮微生物群落丰度和组成特征[J]. 植物营养与肥料学报, 2021, 27(5):741-750.doi:10.11674/zwyf.20514.
	Wang C, Chen J, Shen R F. Diazotroph abundance and community composition in different parts of maize(Zeamays L.) cultivated in acid red soil[J]. Journal of Plant Nutrition and Fertilizers, 2021, 27(5):741-750.
[44]	Gao M, Zhou J J, Wang E T, Chen Q, Xu J, Sun J G. Multiphasic characterization of a plant growth promoting bacterial strain,Burkholderia sp.7016 and its effect on tomato growth in the field[J]. Journal of Integrative Agriculture, 2015, 14(9):1855-1863.doi:10.1016/S2095-3119(14)60932-1. URL
[45]	Issifu M, Songoro E K, Onguso J, Ateka E M, Ngumi V W. Potential of Pseudarthrobacter chlorophenolicus BF2P4-5 as a biofertilizer for the growth promotion of tomato plants[J]. Bacteria, 2022, 1(4):191-206.doi:10.3390/bacteria1040015. URL
[46]	Tang S, Zhou J J, Pan W K, Sun T, Liu M J, Tang R, Li Z J, Ma Q X, Wu L H. Effects of combined application of nitrogen,phosphorus,and potassium fertilizers on tea(Camellia sinensis)growth and fungal community[J]. Applied Soil Ecology, 2023, 181:104661.doi:10.1016/j.apsoil.2022.104661. URL
[47]	何应会, 黄耀恒, 陆荣民, 杨日升, 韦燕妮, 梁文汇. 油梨根际土壤微生物群落及其共生网络对根腐病的响应[J]. 中南林业科技大学学报, 2024, 44(4):106-115.doi:10.14067/j.cnki.1673-923x.2024.04.012.
	He Y H, Huang Y H, Lu R M, Yang R S, Wei Y N, Liang W H. Responses of avocado rhizosphere soil microbial community and its co-occurrence network to root rot disease[J]. Journal of Central South University of Forestry & Technology, 2024, 44(4):106-115.
[48]	Guo B, Zhang L, Sun H J, Gao M J, Yu N, Zhang Q Y, Mou A Q, Liu Y. Microbial co-occurrence network topological properties link with reactor parameters and reveal importance of low-abundance genera[J]. npj Biofilms and Microbiomes, 2022, 8:3.doi:10.1038/s41522-021-00263-y. pmid: 35039527
[49]	Liang J L, Liu J, Jia P, Yang T T, Zeng Q W, Zhang S C, Liao B, Shu W S, Li J T. Novel phosphate-solubilizing bacteria enhance soil phosphorus cycling following ecological restoration of land degraded by mining[J]. The ISME Journal, 2020, 14(6):1600-1613.doi:10.1038/s41396-020-0632-4. URL
[50]	Liu W B, Ling N, Luo G W, Guo J J, Zhu C, Xu Q C, Liu M Q, Shen Q R, Guo S W. Active phoD-harboring bacteria are enriched by long-term organic fertilization[J]. Soil Biology and Biochemistry, 2021, 152:108071.doi:10.1016/j.soilbio.2020.108071. URL
[51]	Metcalf W W, Wanner B L. Involvement of the Escherichia coli phn(psiD)gene cluster in assimilation of phosphorus in the form of phosphonates,phosphite,Pi esters,and Pi[J]. Journal of Bacteriology, 1991, 173(2):587-600.doi:10.1128/jb.173.2.587-600.1991. pmid: 1846145
[52]	Li Q, Zhang H W, Song Y, Wang M Y, Hua C C, Li Y S, Chen S F, Dixon R, Li J L. Alanine synthesized by alanine dehydrogenase enables ammonium-tolerant nitrogen fixation in Paenibacillus sabinae T27[J]. Proceedings of the National Academy of Sciences of the United States of America, 2022, 119(49):e2215855119.doi:10.1073/pnas.2215855119.

序号 Number	菌株 Strain	菌落特征 Colony characteristics	最高相似性菌株 Similarity with known species	相似性/% Similarity
M3	白色芽孢杆菌	圆形,白色,表面干燥有褶皱	B.albus N35-10-2^T	99.86
M4	高地芽孢杆菌	圆形,米黄色,表面光滑湿润	B.altitudinis 41KF2b^T	99.93
M7	巨大芽孢杆菌	圆形,黄白色,平隆,表面略有光泽	B.megaterium NBRC 15308^T	100.00
M8	阿氏芽孢杆菌	圆形,米白色,菌落扁平,较大	B.aryabhattai B8W22^T	100.00
M9	安徽黄杆菌	圆形,黄色,表面有凸起	F.anhuiense D3^T	99.71
M10	东洋芽孢杆菌	圆形,白色,菌落扁平,边缘波纹状	B.toyonensis BCT-7112^T	99.93
M11	霍氏假单胞菌	圆形,白色,表面光滑湿润	P.rhodesiae CIP 104664^T	99.86
M12	吲哚氧化假单胞菌	圆形,边缘整齐,黄色,湿润,中央凸起	P.indoloxydans IPL-1^T	98.94
M17	蜡样芽孢杆菌	圆形,白色,表面干燥有褶皱	B.cereus ATCC 14579^T	99.72
M20	约氏不动杆菌	圆形,边缘瓣状,米白色,中央凸起,湿润	A.johnsonii ATCC 17909^T	99.51
M21	威兹曼芽孢杆菌	圆形,边缘整齐,乳白色	B.wiedmannii FSL W8-0169^T	99.88
M27	利雅得窄食单胞菌	圆形,米白色,菌落扁平,较大	S.riyadhensis CFS3442^T	99.85
M28	巨大芽孢杆菌	圆形,黄白色,平隆,表面略有光泽	B.megaterium NBRC 15308^T	99.93
M52	根际假单胞菌	圆形,边缘整齐,乳白色,表面光滑	P.rhizophila s211^T	99.93
M59	多粘芽孢杆菌	圆形,白色,表面光滑湿润	Pae.polymyxa ATCC 842^T	99.36
M65	人参土黄杆菌	圆形,边缘整齐,黄色,湿润,中央凸起	F.ginsengisoli DCY54^T	97.95
M169	水原微杆菌	圆形,边缘整齐,淡黄色,湿润,表面有凸起	M.suwonense M1T8B9^T	99.28
M182	特基拉芽孢杆菌	不规则圆形,白色,表面干燥有褶皱	B.tequilensis KCTC 13622^T	99.99
M211	环状尼尔菌	椭圆形,灰白色,表面光滑湿润	N.circulans ATCC 4513^T	99.44
M228	解淀粉芽孢杆菌	圆形,淡黄色,菌落不透明,表面有凸起	B.amyloliquefaciens NBRC 15535^T	100.00
M244	安徽黄杆菌	圆形,黄色,表面有凸起	F.anhuiense D3^T	99.85
M245	暹罗芽孢杆菌	圆形,乳白色,表面干燥有褶皱	B.siamensis KCTC 13613^T	99.86
M276	铜绿假单胞菌	不规则圆形,米白色,边缘不整齐,湿润	P.aeruginosa JCM 5962^T	99.79
M325	荧光假单胞菌	圆形,淡黄色,表面湿润有隆起	P.fluorescens F113^T	99.30
M339	墨西哥芽孢杆菌	圆形,浅黄色,湿润,中央凸起	B.mexicanus FSQ1^T	99.84
M427	贝莱斯芽孢杆菌	圆形,淡黄色,边缘整齐	B.velezensis CR-502^T	99.93

序号 Number	菌株 Strain	菌落特征 Colony characteristics	最高相似性菌株 Similarity with known species	相似性/% Similarity
M3	白色芽孢杆菌	圆形,白色,表面干燥有褶皱	B.albus N35-10-2^T	99.86
M4	高地芽孢杆菌	圆形,米黄色,表面光滑湿润	B.altitudinis 41KF2b^T	99.93
M7	巨大芽孢杆菌	圆形,黄白色,平隆,表面略有光泽	B.megaterium NBRC 15308^T	100.00
M8	阿氏芽孢杆菌	圆形,米白色,菌落扁平,较大	B.aryabhattai B8W22^T	100.00
M9	安徽黄杆菌	圆形,黄色,表面有凸起	F.anhuiense D3^T	99.71
M10	东洋芽孢杆菌	圆形,白色,菌落扁平,边缘波纹状	B.toyonensis BCT-7112^T	99.93
M11	霍氏假单胞菌	圆形,白色,表面光滑湿润	P.rhodesiae CIP 104664^T	99.86
M12	吲哚氧化假单胞菌	圆形,边缘整齐,黄色,湿润,中央凸起	P.indoloxydans IPL-1^T	98.94
M17	蜡样芽孢杆菌	圆形,白色,表面干燥有褶皱	B.cereus ATCC 14579^T	99.72
M20	约氏不动杆菌	圆形,边缘瓣状,米白色,中央凸起,湿润	A.johnsonii ATCC 17909^T	99.51
M21	威兹曼芽孢杆菌	圆形,边缘整齐,乳白色	B.wiedmannii FSL W8-0169^T	99.88
M27	利雅得窄食单胞菌	圆形,米白色,菌落扁平,较大	S.riyadhensis CFS3442^T	99.85
M28	巨大芽孢杆菌	圆形,黄白色,平隆,表面略有光泽	B.megaterium NBRC 15308^T	99.93
M52	根际假单胞菌	圆形,边缘整齐,乳白色,表面光滑	P.rhizophila s211^T	99.93
M59	多粘芽孢杆菌	圆形,白色,表面光滑湿润	Pae.polymyxa ATCC 842^T	99.36
M65	人参土黄杆菌	圆形,边缘整齐,黄色,湿润,中央凸起	F.ginsengisoli DCY54^T	97.95
M169	水原微杆菌	圆形,边缘整齐,淡黄色,湿润,表面有凸起	M.suwonense M1T8B9^T	99.28
M182	特基拉芽孢杆菌	不规则圆形,白色,表面干燥有褶皱	B.tequilensis KCTC 13622^T	99.99
M211	环状尼尔菌	椭圆形,灰白色,表面光滑湿润	N.circulans ATCC 4513^T	99.44
M228	解淀粉芽孢杆菌	圆形,淡黄色,菌落不透明,表面有凸起	B.amyloliquefaciens NBRC 15535^T	100.00
M244	安徽黄杆菌	圆形,黄色,表面有凸起	F.anhuiense D3^T	99.85
M245	暹罗芽孢杆菌	圆形,乳白色,表面干燥有褶皱	B.siamensis KCTC 13613^T	99.86
M276	铜绿假单胞菌	不规则圆形,米白色,边缘不整齐,湿润	P.aeruginosa JCM 5962^T	99.79
M325	荧光假单胞菌	圆形,淡黄色,表面湿润有隆起	P.fluorescens F113^T	99.30
M339	墨西哥芽孢杆菌	圆形,浅黄色,湿润,中央凸起	B.mexicanus FSQ1^T	99.84
M427	贝莱斯芽孢杆菌	圆形,淡黄色,边缘整齐	B.velezensis CR-502^T	99.93

序号 Number	固氮能力 Nitrogen- fixing capacity (SI)	产铁载体能力 Siderophore capacity (SI)	溶有机磷能力 Organo- phosphates solubility (SI)	IAA产量/ (mg/L) IAA production
M3	—	—	—	—
M4	1.50	—	1.33	—
M7	1.81	—	—	18.18
M8	—	—	1.09	17.25
M9	—	—	—	17.30
M10	1.75	—	—	—
M11	2.33	1.72	2.81	18.48
M12	1.29	—	—	—
M17	—	—	—	—
M20	—	—	—	—
M21	—	—	—	—
M27	—	—	—	16.09
M28	2.21	—	—	18.62
M52	—	—	—	—
M59	—	—	1.12	—
M65	—	—	—	—
M169	—	—	—	—
M182	—	—	—	—
M211	—	—	—	17.14
M228	—	—	—	—
M244	—	—	—	16.16
M245	1.44	—	—	—
M276	—	—	1.89	17.16
M325	1.13	—	—	16.77
M339	—	—	—	—
M427	1.85	1.42	1.51	15.84

序号 Number	固氮能力 Nitrogen- fixing capacity (SI)	产铁载体能力 Siderophore capacity (SI)	溶有机磷能力 Organo- phosphates solubility (SI)	IAA产量/ (mg/L) IAA production
M3	—	—	—	—
M4	1.50	—	1.33	—
M7	1.81	—	—	18.18
M8	—	—	1.09	17.25
M9	—	—	—	17.30
M10	1.75	—	—	—
M11	2.33	1.72	2.81	18.48
M12	1.29	—	—	—
M17	—	—	—	—
M20	—	—	—	—
M21	—	—	—	—
M27	—	—	—	16.09
M28	2.21	—	—	18.62
M52	—	—	—	—
M59	—	—	1.12	—
M65	—	—	—	—
M169	—	—	—	—
M182	—	—	—	—
M211	—	—	—	17.14
M228	—	—	—	—
M244	—	—	—	16.16
M245	1.44	—	—	—
M276	—	—	1.89	17.16
M325	1.13	—	—	16.77
M339	—	—	—	—
M427	1.85	1.42	1.51	15.84

处理 Treatments	株高/cm Plant height	地上部鲜质量/g Fresh weight of above ground	地上部干质量/g Dry weight of above ground	根部鲜质量/g Fresh weight of roots	根部干质量/g Dry weight of roots
CK	50.15±0.77d	5.57±0.20c	0.70±0.05c	1.33±0.12b	0.27±0.02b
M11_5	58.15±0.35b	6.62±0.07b	0.91±0.02ab	1.79±0.13a	0.33±0.01b
M11_7	61.45±0.93a	7.57±0.22a	0.95±0.04a	1.93±0.06a	0.40±0.01a
M11_9	54.13±1.33c	6.25±0.22b	0.82±0.02b	1.46±0.09b	0.31±0.02b

Please choose a citation manager

Content to export