Effects of Low Temperature Irrigation at Booting Stage on Physiological Characteristics of Sword Leaves and Spikelets in Rice

doi:10.7668/hbnxb.20190947

Abstract

Abstract: To explore persistent damage mechanism of low temperature on rice during the booting stage, this experiment used four rice varieties widely used in large areas of production:Yliangyou 957, Tianyouhuazhan, Yliangyou 1, Zhongzao 39. Respectively in their panicle differentiation Ⅴ period with 17.5℃ cold water irrigation, continued treatment for 10 days. After 10 days of treatment, SPAD value of sword leaves was measured by SPAD502. Physiological and biochemical indicators such as the permeation regulation system index and antioxidant system index of sword leaves and spikelets were measured by Elisa kit. ABA content of spikelets was measured by high performance liquid chromatography tandem mass spectrometry. The results showed that low temperature stress at booting stage reduced rice seed setting rate and pollen fertility. This difference in the reduction of both parameters was observed among the four rice varieties. It suggested that one of the principal reasons for the decline in setting rate was the decline in pollen fertility. The SPAD value of sword leaves decreased after low temperature stress during booting stage, and different varieties showed different degrees of decline. According to the results of the setting rate, Yliangyou 957 and Tianyouhuazhan could be considered as low temperature tolerant varieties while Yliangyou 1 and Zhongzao 39 could be considered as low temperature sensitive varieties. After low temperature treatment, SOD, POD and CAT activities were increased in the leaves of the tolerant varieties, while SOD activities were decreased in spikelets. SOD and POD activities of sword leaves of sensitive varieties were decreased, which was contrary to that in low temperature tolerant cultivars. Osmotic regulators in the low temperature tolerant varieties were mostly higher than those in the control group, and osmotic regulators in the spikelets were lower than those in the control group. The low temperature sensitive cultivars showed the opposite trend. ABA content in spikelets was significantly higher than that in control group, regardless of whether the varieties is resistant to low temperature. Spikelets and sword leaves were mostly showing the opposite trend on physiological and biochemical indicators. It included the SOD activity of the antioxidant system, PRO and STS content of the osmosis control system, and so on. It was speculated that there might be a dynamic regulation mechanism of the rice source sink relationships under low temperature stress, which provided a new idea for the research on the effects of low temperature stress on rice physiology at the booting stage.

Key words: Rice, Booting stage, Low temperature, Physiology, Spikelets

CLC Number:

S511.01

LUO Qiuhong, WU Jun, BAI Bin, ZHUANG Wen, YAO Dongping, XIAO Gui, LIU Xionglun, DENG Qiyun. Effects of Low Temperature Irrigation at Booting Stage on Physiological Characteristics of Sword Leaves and Spikelets in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 111-118. doi: 10.7668/hbnxb.20190947.

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