Abstract: In the study, the alternative splicing variants of new HD2 protein(OsHDT703)were identified by bioinformatics analysis combining with RT-PCR, TA cloning and sequencing technology. A new HD2 family protein(OsHDT703)was detected by bioinformatics analysis, which contained the NPL conserved domain at N-terminus. Sixteen HD2 proteins were chosen from seven species to construct evolutionary tree. The results showed that OsHDT703 protein was in the same branch as OsHDT702 protein, and OsHDT701 was in another branch, indicating that HD2 family proteins might have the functional differentiation in rice. Four specific primers were designed based on different alternative splicing variants, and the cDNA of rice leaves was used as template. At least four alternative splicing variants of OsHDT703 were cloned by RT-PCR and sequencing. Because the CDS sequences of OsHDT703.3 and OsHDT703.4 were the same, indicating that OsHDT703 had at least 4 different alternative splicing variants. In summary, a new HD2 protein was identified in rice, named as OsHDT703, and at least four alternative splicing variants were identified, which laid the foundation for further study on the of OsHDT703 function.

Key words: Rice, Histone deacetylase(HDAC), HD2 protein family, Alternative splicing, Bioinformatics

CLC Number: 

• S511.03