Analysis of Differentially Expressed Genes in Organs of Lilium formolongi Julius

doi:10.7668/hbnxb.2017.05.005

Abstract

Abstract: To understand the expression levels of 17 candidate genes in flower,leaf and scale of Lilium formolongi Jilius,Real time quantitative PCR was conducted. The results showed that the 17 candidate genes could be divided into five groups.CL10636.Contig1_All,CL6300.Contig2_All,CL7951. Contig5_All,Unigene8314_All,Unigene19279_All and CL1306.Contig1_All significantly highly expressed in flowers,which were highly homologous with Bidirectional sugar transporter SWEET4-like gene,cold-regulated gibberellin-regulated protein gene bidirectional,mitochondrial chaperone gene,Lightlight-dependent short hypocotyls 4-like gene,1-deoxyxylulose-5-phosphate synthase gene,geranylgeranyl pyrophosphate synthase gene. CL9292.Contig2_All,CL212.Contig5_All,Unigene1800_All,CL3468. Contig1_All,CL4520.Contig5_All,Unigene4212_All,CL4079.Contig1_All and Unigene10210_All eight significantly highly expressed in leaves,which were highly homologous with GDSL esterase/lipase gene,flavonoid O-methyltransferase gene,hydroperoxide lyase gene,4-hydroxy-3-methylbut-2-en-1-yl diphosphate gene,polyphenol oxidase gene,glucomannan 4-beta-mannosyltransferase 1-like gene,Levopimaradiene synthase gene and trans-ocimene synthase gene. CL2405.Contig1_All significantly highly expressed in scales,function unknown. CL1114.Contig2_All significantly highly expressed in both flowers and leaves,highly homologous with 3-ketoacyl-CoA thiolase gene. CL1772.Contig2_All,homologous with delta-(24)-sterol reductase,was non-organ specific gene. The results are valuable for the functional study of those genes and will be benefit for the molecular breeding of lily.

Key words: Lily, Differentially expressed genes, Real-time q-PCR, Organs

CLC Number:

S682.29
Q78

DU Fang, WANG Ting, FAN Junmiao, ZHANG Haoyu. Analysis of Differentially Expressed Genes in Organs of Lilium formolongi Julius[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(5): 25-30. doi: 10.7668/hbnxb.2017.05.005.

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References

[1] 陈敏敏,张建军,周音,等. 百合根外植体体细胞胚胎再生影响因素研究[J]. 核农学报,2015,29(8):1494-1501.
[2] 钟军珺. 榆叶梅花发育相关MADs-box基因克隆和表达模式研究[D]. 北京:北京林业大学,2016:2-7.
[3] 张雪梅,赵婧,赵银河. 莲瓣兰大雪素SEP1 基因克隆和表达的研究[J]. 种子,2016,35(4):9-12.
[4] 徐静,王莉,钱前,等. 水稻叶片形态建成分子调控机制研究进展[J]. 作物学报,2013,39(5):767-774.
[5] Chen S,Luo H,Li Y,et al. 454 EST analysis detects genes putatively involved in ginsenoside biosynthesis in Panax ginseng[J]. Plant Cell Reports,2011,30(9):1593-1601.
[6] Fan X D,Wang J Q,Yang N,et al. Gene expression profiling of soybean leaves and roots under salt,saline-alkali and drought stress by high-throughput Illumina sequencing[J]. Gene,2013,512(2):392-402.
[7] Villacorta-Martin C,Gonzalez F F,Huijben K,et al. Whole transcriptome profiling of the vernalization process in Lilium longiflorum(cultivar White Heaven) bulbs[J]. BMC Genomics,2015,16:1-16.
[8] Wang J,Wang Q,Yang Y,et al. De novo assembly and characterization of stress transcriptome and regulatory networks under temperature,salt and hormone stresses in Lilium lancifolium[J]. Molecular Biology Report,2014,41(12):8231-8245.
[9] Li X E,Wang C X,Cheng J Y,et al. Transcriptome analysis of carbohydrate metabolism during bulblet formation and development in Lilium davidii var. unicolor[J]. BMC Plant Biology,2014,14:358.
[10] Zhu X P,Chai M,Li Y,et al. Global transcriptome profiling analysis of inhibitory effects of paclobutrazol on leaf growth in lily (Lilium longiflorum-Asiatic Hybrid)[J]. Frontiers in Plant Science,2016,7:491.
[11] Lang V,Usadel B,Obermeyer G. De novo sequencing and analysis of the lily pollen transcriptome:an open access data source for an orphan plant species[J]. Plant Molecular Biology,2015,87(1/2):69-80.
[12] Liu X H,Huang J,Wang J M,et al. RNA-Seq analysis reveals genetic bases of the flowering process in oriental hybrid lily cv. Sorbonne[J]. Russian Journal of Plant Physiology,2014,61(6):880-892.
[13] Rhee S J,Seo M,Jang Y J,et al. Transcriptome profiling of differentially expressed genes in floral buds and flowers of male sterile and fertile lines in watermelon[J]. BMC Genomics,2015,16:914.
[14] Zhang M F,Jiang L M,Zhang D M,et al. De novo transcriptome characterization of Lilium Sorbonne and key enzymes related to the flavonoid biosynthesis[J]. Molecular Genetics and Genomics,2015,290(1):399-412.
[15] Du F,Wu Y,Zhang L,et al. De novo assembled transcriptome analysis and SSR marker development of a mixture of six tissues from Lilium Oriental Hybrid Sorbonne[J]. Plant Molecular Biology Report,2014,33(2):281-293.
[16] 杜方. 百合不同器官转录组分析及SSR标记开发应用[D]. 杭州:浙江大学,2014:24-25.
[17] 王玉倩,薛秀花. 实时荧光定量PCR技术研究进展及其应用[J]. 生物学通报,2006,51(2):1-5.
[18] 刘畅,姜晶,韩晓雪,等. 植物中SWEET基因家族研究进展[J]. 植物生理学报,2014,50(9):1367-1373.
[19] Tamai S,Iida H,Yokota S,et al. Characterization of the mitochondrial protein LETM1,which maintains the mitochondrial tubular shapes and interacts with the AAA-ATPase BCS1L[J]. Journal of Cell Science,2008,121(15):2588-2600.
[20] Takeda S,Hanano K,Kariya A,et al. CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3,two members of the ALOG gene family,in shoot organ boundary cells[J]. Plant Journal,2011,66(6):1066-1077.
[21] Muhlemann J K,Klempien A,Dudareva N. Floral volatiles:from biosynthesis to function[J]. Plant Cell and Environment,2014,37(SI):1936-1949.
[22] Chepyshko H,Lai C P,Huang L M,et al. Multifunctionality and diversity of GDSL esterase/lipase gene family in rice(Oryza sativa L.japonica)genome:new insights from bioinformatics analysis[J]. BMC Genomics,2012,13(1):1-19.
[23] Keeling C I,Madilao L L,Zerbe P A,et al. The primary diterpene synthase products of picea abies levopimaradiene/abietadiene synthase (PaLAS) are epimers of a thermally unstable diterpenol[J]. Journal of Biological Chemistry,2011,286(24):21145-21153.
[24] Kim J H,Lee K I,Chang Y J,et al. Developmental pattern of Ginkgo biloba levopimaradiene synthase (GbLPS) as probed by promoter analysis in Arabidopsis thaliana[J]. Plant Cell Reports,2012,31(6):1119-1127.
[25] Ro D K,Bohlmann J. Diterpene resin acid biosynthesis in loblolly pine(Pinus taeda):functional characterization of abietadiene/levopimaradiene synthase(PtTPS-LAS)cDNA and subcellular targeting of PtTPS-LAS and abietadienol/abietadienal oxidase(PtAO,CYP720B1)[J]. Phytochemistry,2006,67(15):1572-1578.
[26] 张传丽,仲月明,沈丹红,等. 植物类黄酮O-甲基转移酶研究进展[J]. 西北植物学报,2012,32(6):1274-1281.
[27] 刘攀峰,杜红岩,杜兰英,等. 1-羟基-2-甲基-2-(E)-丁烯基-4-二磷酸合酶基因cDNA全长克隆与序列分析[J]. 植物研究,2012(4):444-451.
[28] 程琪庆,何云飞,李耿,等. 丹参4-羟基-3-甲基-2-丁烯基焦磷酸还原酶基因的全长克隆与诱导表达分析[J]. 药学学报,2013(2):236-242.
[29] 王曼玲,胡中立,周明全,等. 植物多酚氧化酶的研究进展[J]. 植物学通报,2005,22(2):215-222.
[30] 谢鑫鑫,吴卫东,林碧英,等. 脂氢过氧化物裂解酶基因对生菜遗传转化的研究[J]. 热带作物学报,2016,37(5):856-861.
[31] Johnson T S,Schwieterman M L,Kim J Y,et al. Lilium floral fragrance:A biochemical and genetic resource for aroma and flavor[J]. Phytochemistry,2016,122:103-112.
[32] Silvestro D,Andersen T G,Schaller H A. Plant sterol metabolism. delta(7)-Sterol-C-5-Desaturase (STE1/DWARF7),delta(5,7)-Sterol-Delta(7)-Reductase (DWARF5) and delta(24)-Sterol-Delta(24)-Reductase (DIMINUTO/DWARF1) show multiple subcellular localizations in Arabidopsis thaliana (heynh) L.[J].PLoS One,2013,8(2):1-11.
[33] 周延清,张永华,张喻,等. 怀地黄3-酮酯酰CoA-硫解酶基因的克隆、序列特征和时空表达分析[J]. 中草药,2013,44(1):76-84.

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