Cloning and Expression Profiles of IbERF3,an Abiotic Stress Related Gene in Sweet Potato

doi:10.7668/hbnxb.2014.06.004

Abstract

Abstract: In order to improve the stress resistance of sweet potato,we used RACE method to clone IbERF3 contained an AP2 domain which belong to ERF superfamily which plays an important role in stress resistance.Blast showed that IbERF3 was a new gene in sweet potato that had never been reported.The cDNA length of IbERF3 was 1 205 bp,and contained an 669 bp open reading frame encoding a 223 amino acids.By homology analysis showed that IbERF3 was conserved in Solanaceae plants.Constitutive expression showed that IbERF3 express in root,leaf and stem,and the expression of IbERF3 was the highest in leaf.The result reveal that the expression of IbERF3 was induced by drought,salt both in leaf and root by qRT-PCR.After treating with ABA,The expression of IbERF3 increased gradually and reached a maximum at 24 h.It suggests that IbERF3 plays significant roles in responses to abiotic stress in sweet potato.

Key words: Sweet potato, ERF, Gene cloning, Expression, Abiotic stress

CLC Number:

Q78
S531.03

BIAN Xiao-feng, XIE Yi-zhi, GUO Xiao-ding, JIA Zhao-dong, MA Pei-yong. Cloning and Expression Profiles of IbERF3,an Abiotic Stress Related Gene in Sweet Potato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(6): 16-20. doi: 10.7668/hbnxb.2014.06.004.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2014.06.004

http://www.hbnxb.net/EN/Y2014/V29/I6/16

References

[1] Kays S J,Kays S E.Sweetpotato chemistry in relation to health [C].Sweetpotato Production Systems toward the 21st Century Kyushu National Agri Expt Sta,Miyakonojo,Miyazaki,Japan,1998:231-272.
[2] 谢一芝,郭小丁,贾赵东,等.紫心甘薯育种现状及展望[J].植物遗传资源学报,2012,13(5):709-713.
[3] 张计育,王庆菊,郭忠仁.植物 AP2/ERF 类转录因子研究进展 [J].遗传,2012,34:835-847.
[4] Mizoi J,Shinozaki K,Yamaguchi-Shinozaki K.AP2/ERF family transcription factors in plant abiotic stress responses[J].Biochim Biophy Acta,2012,1819(2):86-96.
[5] Nakano T,Suzuki K,Fujimura T,et al.Genome-wide analysis of the ERF gene family in Arabidopsis and rice[J].Plant Physiology,2006,140(2):411-432.
[6] Park J M,Park C J,Lee S B,et al.Overexpression of the tobacco Tsi1 gene encoding an EREBP/AP2-type transcription factor enhances resistance against pathogen attack and osmotic stress in tobacco[J].The Plant Cell,2001,13(5):1035-1046.
[7] Guo Z J,Chen X J,Wu X L,et al.Overexpression of the AP2/EREBP transcription factor OPBP1 enhances disease resistance and salt tolerance in tobacco[J].Plant Molecular Biology,2004,55(4):607-618.
[8] Huang Z J,Zhang Z J,Zhang X L,et al.Tomato TERF1 modulates ethylene response and enhances osmotic stress tolerance by activating expression of downstream genes[J].FEBS Letters,2004,573(1/3):110-116.
[9] Zhang H,Huang Z,Xie B,et al.The ethylene-,jasmonate-,abscisic acid- and NaCl-responsive tomato transcription factor JERF1 modulates expression of GCC box-containing genes and salt tolerance in tobacco[J].Planta,2004,220(2):262-270.
[10] Lee J H,Hong J P,Oh S K,et al.The ethylene-responsive factor like protein 1 (CaERFLP1) of hot pepper (Capsicum annuum L.) interacts in vitro with both GCC and DRE/CRT sequences with different binding affinities:possible biological roles of CaERFLP1 in response to pathogen infection and high salinity conditions in transgenic tobacco plants [J].Plant Molecular Biology,2004,55:61-81.
[11] Yi S Y,Kim J H,Joung Y H,et al.The pepper transcription factor CaPF1 confers pathogen and freezing tolerance in Arabidopsis[J].Plant Physiology,2004,136(1):2862-2874.
[12] Kim Y H,Jeong J C,Park S,et al.Molecular characterization of two ethylene response factor genes in sweetpotato that respond to stress and activate the expression of defense genes in tobacco leaves[J].Journal of Plant Physiology,2012,169(11):1112-1120.
[13] Solano R,Stepanova A,Chao Q,et al.Nuclear events in ethylene signaling:a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPON-SE-FACTOR1[J].Genes & Development,1998,12(23):3703-3714.
[14] Berrocal-Lobo M,Molina A,Solano R.Constitutive expression of ETHYLENE-RESPONSE-FACTOR1 in Arabidopsis confers resistance to several necrotrophic fungi[J].The Plant Journal :for Cell and Molecular Biology,2002,29(1):23-32.
[15] 朱旗,徐吉臣.植物抗旱分子机制研究进展 [J].安徽农业科学,2010(26):14198-14202.

Please choose a citation manager

Content to export