Prokaryotic Expression Vector Construction and Expression of Prunus persica PpPAE

doi:10.7668/hbnxb.2014.02.004

Abstract

Abstract: This study was aimed to construct prokaryotic expression vector pET-32a (+ ) -PpPAE and obtain high expression of PpPAE fusion protein in E. coli. cDNA sequence of PpPAE(GenBank No. KF017595) was ampli- fied and constructed into the prokaryotic expression vector pET-32a. Then the recombinant plasmid was transformed into E. coli BL21 (DE3) cell. The fusion protein was induced by IPTG and analyzed by SDS-PAGE. The result re- vealed that the prokaryotic expression vector of pET-32a (+ ) -PpPAE was constructed successfully. The fusion pro- tein of about 64 kDa was highly expressed and accumulated after induction with 0. 1 mmol /L IPTG for 4 h. This work laid the foundation for further study of Prunus persica PpPAE.

Key words: Prunus persica, Pectin acetylesterase (PAE), Prokaryotic expression

CLC Number:

S662.1
Q78

CONG Li-jun, WANG Bin, DUAN Yan-xin, WU Jun-shuai, FENG Jing. Prokaryotic Expression Vector Construction and Expression of Prunus persica PpPAE[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(2): 18-21. doi: 10.7668/hbnxb.2014.02.004.

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