Cloning and Characterization of the MYB Gene GmMYBJ7from Soybean

doi:10.7668/hbnxb.2011.05.022

Abstract

Abstract: The MYB proteins play an important role in plant,which involved in the regulation of the process of metabolism and development extensively.The function of MYB transcription factor gene GmMYBJ7,which was isolated from soybean using the method of RACE-PCR,was to be clarified preliminarily in this paper.The expression pattern of GmMYBJ7 in different organs was studied using Northen blot.The results showed that the expression of GmMYBJ7 was detected in the leaves and stems.The transcriptional activation ability of GmMYBJ7 protein was confirmed by the yeast system with β-galactosidase activity of 24.31 U.Semi-quantitative RT-PCR analysis indicated that GmMYBJ7 may also repress the expression of some flavonoid biosynthetic genes,such as PAL(Phenylalanine ammonia lyase),C4H(cinnamate-4-hydroxylase),4CL(4-coumaroyl-CoA ligase),CHS(Chalcone Synthase),CHI(chalcone isomerase)and F3H(flavanone 3-hydroxylase),which is considered to be related to flavonoid biosynthesis in palnt.

Key words: Soybean, MYB transcription factors, Gene expression and regulation

CLC Number:

S565.1

YANG Wen-jie, WU Yan-min, TANG Yi-xiong. Cloning and Characterization of the MYB Gene GmMYBJ7from Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(5): 107-111. doi: 10.7668/hbnxb.2011.05.022.

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