Studies on Methanogens Composition of Inner Mongolia Casmere Goat Rumen in Different Feeding Condition

doi:10.7668/hbnxb.2010.05.047

Abstract

Abstract: In order to analyze the methanogen species composition in the rumen from grazing and barn feeding cashmere goat respectively. A pair of mcrA gene specific primers was designed,and total microbial genomic DNA of rumen liquid was extracted by the direct method of PCR-amplification template. Then a mcrA gene specific DNA clone library was subsequently constructed. And RFLP analysis of mcrA gene was conducted using enzymes(Taq I) to digest mcrA gene from the library. The randomly selected cloned fragments were finally clustered into 6 different phylotypes, and contruct the DNA fingerprint patterns from it. Through the phylogenetic trees analyzing,Methanogen in cashmere goat rumen belong to methanobacteriales and methanomicrobiales,and the preponderant methanogen was methanobrevibacter. The methanogen in grazing cashmere goat rumen was more abundant than the one in barnbreeding. The grazing cashmere goat had a higher proportion of six kinds of methanogen than the barn feeding cashmere goat,the latter only have two kinds of methanogen which were higher proportion,the others were low proportion.

Key words: Methanogen, mcrA gene, Rumen liquid, Cashmere goat

CLC Number:

S827

LI Changqing, JIN Hai, Mitsunori KURIHARA. Studies on Methanogens Composition of Inner Mongolia Casmere Goat Rumen in Different Feeding Condition[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 228-233. doi: 10.7668/hbnxb.2010.05.047.

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