Screening and Identification of the Probiotics Strain Y5-39 Showing Antidysenteriae Effects from Sheep

doi:10.7668/hbnxb.2010.05.042

Abstract

Abstract: The research was to screening the probiotics curing diarrhea of lamb. A strain Y5-39 which had strong inhibition capability against Eacterium coli was obtained with the agar plate diffusion method from dung of healthy Han Sheep. Then the strain Y5-39 was characterized by morphological and culture features observation,physiological and biochemical experiments and 16 S rDNA sequence analysis. As the morphology characteristics and physiological and biochemical characteristics of the stain was similar to Bacillus and the similarity of the 16S rDNA sequences between strain Y5-39 and the type strain was up to 99. 92% . According to the results,strain Y5-39 was finally identified as Bacillus licheniformis. The survival rates of bacteria after being inoculated in artificial gastric juice,acid and bile salt environment for different time was determined. The test results showed that Y5-39 showed stronger tolerance in bile salt,artificial gastric juice and acid environment.

Key words: Sheep, Probiotics, Identification, Tolerance

CLC Number:

S646

GUO Yunxia, HAO Qinghong, ZHU Baocheng. Screening and Identification of the Probiotics Strain Y5-39 Showing Antidysenteriae Effects from Sheep[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 206-210. doi: 10.7668/hbnxb.2010.05.042.

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