Effect of Exogenous H2O2 on Photosynthetic Characteristics in PEPC Transgenic Rice

doi:10.7668/hbnxb.2010.04.028

Abstract

Abstract: To elucidate the physiological mechanism of transgenic rice over expressing C₄ photosynthetic genes responds to peroxide stress，the photosynthetic characteristics of the functional leaves net photosynthetic rate Pn，stomatal conductance Gs，intercellular CO₂ concentration Ci，transpiration rate Tr and water utilization rate from PEPC transgenic rice and its wild type Kitaake were studied with Li-6400 portable photosynthesis system under different density exogenous H₂O₂. Net photosynthetic rate of transgenic rice treated with 0.5－5 mmol /L H₂O₂ descend as same as changing of stomatal conductance compared to control，and the inhibition are weaken as light intensity increasing. Stomatal conductance of transgenic rice under 50 mmol /L H₂O₂ treatment is raise but net photosynthetic rate is unchange. Photosynthesis physiological nature of Kitaake under H₂O₂ concentration gradient are not characteristic like transgenic rice. So 0.5 mmol /L is effective density to regulate stomatal conductance of transgenic rice，to affect photosynthesis physiological characteristic.

Key words: Rice, C₄ PEPC gene, Net photosynthetic rate, Stomatal conductance

CLC Number:

S511.01

REN Cheng-gang, LI Xia. Effect of Exogenous H2O2 on Photosynthetic Characteristics in PEPC Transgenic Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 130-135. doi: 10.7668/hbnxb.2010.04.028.

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http://www.hbnxb.net/EN/Y2010/V25/I4/130

References

[1] 焦德茂,李霞,黄雪清,等.转PEPC基因水稻的光合CO2同化和叶绿素莹光特性[J].科学通报,2001,46(5)414-418.

[2] Ku M S B,Agarie S,Nomura M,et al.High-level expression of maize phosphoenolpyruvate carboxylase in transgenic rice plants[J].Nature Biotechnol,1999,l7:76-80.

[3] Fukayama H,Tamai T,Tsuchida H,et al.Effects of overproduction of the maize C4-specific phosphoenolpyruvate carboxylase on photosynthesis[J].CSIRO publishing Brisbane,Astralia,2001,S17-012:1-4

[4] 黄雪清,焦德茂,迟伟,等.转C4酶基因水稻的CO2交换和莹光特性[J].植物学报,2002,44(4):405-412.

[5] 焦德茂,匡廷云,李霞,等.转PEPC基因水稻具有初级CO2 浓缩机制性理特点[J].中国科学,2003,33(3):438-446.

[6] 王仁雷,华春,李霞,等.光抑制条件下转PEPC基因水稻的光合表现[J].作物学报,2002,28(5):321-326.

[7] 李霞,焦德茂,戴传超.转PEPC基因水稻对光氧化逆境的响应[J].作物学报,2005b,31(4):408-413.

[8] Foreman J,Demidchik V,Bothwell J H,et al.Reactive oxygen species produced by NADPH oxidase regulate plant cell growth[J].Nature,2003,422:442-446.

[9] Li J,Stouffs M,Serrander L,et al.The NADPH oxidase NOX4 drives cardiac differentiation:role in regulating cardiac transcription factors and MAP kinase activation[J].Mol Biol Cell,2006,17:3978-3988.

[10] Cai H.Hydrogen peroxide regulation of endothelial function:origins mechanisms and consequences[J].Cardiovasc Res,2005,68:26-36.

[11] Rizhsky L.Double antisense plants lacking ascorbate peroxidase and catalase are less sensitive to oxidative stress than single antisense plants lacking ascorbate per.oxidase or catalase[J].Plant J,2002,32:329-342.

[12] Steven Neill,Radhika Desikan,John Hancock.Hydrogen peroxide signalling[J].Current Opinion in Plant Biology,2002(5):388-395.

[13] 李霞,吴爽,焦德茂,等.转PEPC基因水稻的选育[J].江苏农业学报,2001,17(3):143-147.

[14] 李霞,刘友良,焦德茂.不同高产水稻品种叶片的荧光参数的日变化和光适应特性的关系[J].作物学报,2002,28(2):145-153.

[15] Zhang X,Zhang L,Dong F C,et al.Hydrogen peroxide is involved in abscisic acid induced stomatal closure in Vicia faba[J].Plant Physiol,2001,126:1438-148.

[16] She X M,Song X G,He J M.Role and relationship of nitric oxide and hydrogen peroxide in light/dark regulated stomatal movement in Vicia faba[J].Acta Bot Sin,2004,46:1292-1300.

[17] 王超,李霞,蔡庆生.不同测定环境条件下转PEPC基因水稻及杂交后代光合特性的比较[J].江苏农业学报,2008,24(3):232-236.

[18] MeKay C P,Hartman H.Hydrogen peroxide and the evolution of oxygenic photosynthesis[J].Orig Life Envol Biosph,1991,21:157-163.

[19] Allan A C,Fluhr R.Two district sources of elicited reactive oxygen species in tobacco epidermal cells[J].Plant Cell,1997,9:1559-1572.

[20] Alvarez M E,Lamb C.Oxidative burst.mediated defense responses in plant disease resistance[C]//Scandalios J G.Oxida-tive Stress and the Molecular Biology of Antioxidant Defenses.New York:Cold Spring Harbor Laboratory Press,1997:815-839.

[21] Karpinski S,Reynolds H,Karpinska B,et al.Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis[J].Science,1999,284:654-657.

[22] Jiang M,Zhang J.Effect of abscisic acid on active oxygen species,antioxidant defencesystem and oxidative damage in leaves of maize seedlings[J].Plant Cell Physiol,2001,42:1265-1273.

[23] Sone J R.An assessment of proposed mechanism for sensing hydrogen peroxide in mammalian systems[J].Arch Biochem Biophys,2004,422:119-124.

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