Cloning of 5' cDNA Ends of Betaine Aldehyde Dehydrogenase from Puccinellia chinampoensis and Construction of Plant Expression Vector

doi:10.7668/hbnxb.2010.02.006

Abstract

Abstract: The sequence of 5' cDNA ends of Betaine-aldehyde Dehydrogenase(BADH)was cloned from Puccinellia chinampoensis by improved RT-PCR and 5' RACE methods.The full length of cDNA was 1 781 bp by overlapping sequence and contained an intact open reading frame(ORF)encoding a putative protein of 506 amino acids.The deduced amino acid sequence contained the conserved motif of aldehyde dehydrogenase which was VT/SLELGGKSP and after it there was the Cys at the 29th site,associated with aldehyde dehydrogenase function.Multiple sequence alignment and phylogenetic analysis showed that the polypeptide shared high homology with Hordeum brevisubulatum BBD1.Then its plant expression vector named PBI121-BADH was constructed.

Key words: Puccinellia chinampoensis, BADH, 5??RACE, GC-rich, Expression vector

CLC Number:

Q785

WANG Lei, ZHONG Ming, GUO Zhi-fu, ZHANG Li, ZHANG Jia, ZHAO Li, LI Hao-ge. Cloning of 5' cDNA Ends of Betaine Aldehyde Dehydrogenase from Puccinellia chinampoensis and Construction of Plant Expression Vector[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 30-34. doi: 10.7668/hbnxb.2010.02.006.

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