Cloning and Construction of RNAi Vector of Flavone Synthase I Gene, BmFNS I from Silkworm,Bombyx mori

doi:10.7668/hbnxb.2009.05.006

Abstract

Abstract: Flavone is widely existing in animals and plants.They have various physiological activity and the synthesization of them can be catalyzed by flavone synthase I gene.Using the reported amino acid sequences of other organisms and the scaffold and EST sequences of silkworm,silkworm flavone synthase I gene,BmFNS I,was obtained by electronic clone.The gene is 1451 bp in length and 7 exons in it by cloning and sequencing.The deduced protein contains 345 amino acids and a structural domain of 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily located in amino acids 179 and 295.It is shown by RT-PCR experiment that the gene expressed mostly in midgut in all the tissues tested.Then gene was subcloned to L4440,which has two T7 polymerase promoter in opposite orientation to synthesize dsRNA in vitro.

Key words: Bombyx mori, BmFNS I, Clone, Tissue expression

CLC Number:

S881.2

KONG Wei-qing, YANG Jin-hong, PENG Yun-wu, HU Bi-li. Cloning and Construction of RNAi Vector of Flavone Synthase I Gene, BmFNS I from Silkworm,Bombyx mori[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(5): 26-30. doi: 10.7668/hbnxb.2009.05.006.

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