Effects of Ethyl Methyl Sulfonate on Physiology of Callus from Leaves of Strawberry

doi:10.7668/hbnxb.2008.03.033

Abstract

Abstract: The calli of strawberry were used as materials to study the effects of Ethyl Methyl Sulfonate(EMS)on the activities of protecting enzyme and oxidizing enzyme,the contents of total phenol and MDA and the death rate of callus.The result showed that with the increase of EMS concentration and treatment time,the death rate of callus increased.With the increase of EMS concentration,the activities of SOD,CAT,PPO and POD increased at first,and decreased sharply afterwards.With the extension of treatment time,SOD activity decreased continuously,activities of CAT and PPO increased first and then decreased,POD activity decreased firstly and then increased,but decreased later. With the increase of EMS concentration and treating time,total phenol contents decreased firstly and then increased,while MDA contents increased continuously. During the culture of callus after EMS treatment,the activities of PPO and POD decreased sharply and then maintained a steady level,contents of total phenol and MDA decreased,and the contents of total phenol was positively correlated with the activities of PPO and POD.It was suggested that the better EMS treatment was 0.1%-0.2% and 1.0-1.5 h,and some measures should be taken for preventing callus browning in the first 12 h after EMS treatment.

Key words: Ethyl methyl sulfonate, Strawberry, Callus, Physiology

CLC Number:

S668.403.6

ZHOU Hou-cheng, LUO Jing, ZHAO Xia, WANG Yong-qing. Effects of Ethyl Methyl Sulfonate on Physiology of Callus from Leaves of Strawberry[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(3): 138-142. doi: 10.7668/hbnxb.2008.03.033.

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