Induced Host Disease by Culture Filtrate of Bipolaris maydis Race C Toxin Cultivation in Maize (Zea mays L.)

doi:10.7668/hbnxb.2008.02.044

Abstract

Abstract: Disease to maize southern leaf spot disease was induced by culture filtrate of Bipolaris maydis race C toxin cultivation.A pair of homokaryon B37 inbred lines in maize was used as the test material.The toxin activity was measured by the detached leaf method to find its optimal concentration as a plant defense activator that induces maize disease resistance,and several enzymes involved in disease resistance were presented.The lesion area on the leaves was significant difference by ANOVA.They were(0.30±0.14) and(0.36±0.17) mm2 respectively after pretreated with 1∶50 and 1∶60 of culture filtrate of Bipolaris maydis race C toxin cultivation were(0.30±0.14) and(0.36±0.17) mm2 respectively.It was decreased 7.5-9 times compared with the CK(2.70±0.24) mm2.At the same time,the changes of peroxidase(POD),phenylalanine ammonialyase(PAL),malondialdenvde(MDA) activities were determined.During 0-72 h inspection,the effect of 1∶60 concentration was better than that of 1∶50,PAL activity increased by 23.4%,POD activity increased by 65.9%,but the malondialdenvde(MDA) content decreased by 28.2% compared with the CK.It was shown that culture filtrate of Bipolaris maydis race C toxin cultivation itself can function as an elicitor to induce host disease resistance in maize.

Key words: Bipolaris maydis race C, Culture filtrate of toxin cultivation, Phenylalanine ammonialyase CPAL), Per-oxidase (POD), Malondialdcnvde MDA (MDA), Irxluced resistance

CLC Number:

S513.01

SHANG Chuang, MA Chun-hong, JIA Yin-suo, ZHAI Cai-xia, LI Yun-chao, DONG Wen-qi, CUI Si-ping, HOU Li-bai. Induced Host Disease by Culture Filtrate of Bipolaris maydis Race C Toxin Cultivation in Maize (Zea mays L.)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(2): 198-204. doi: 10.7668/hbnxb.2008.02.044.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2008.02.044

http://www.hbnxb.net/EN/Y2008/V23/I2/198

References

[1] Hooke A L,Smith D R,Lim S M,et al.Reaction of corn seeding with male-sterile cytoplasm to Helminthosporium maydis[J].Plant Dis.Rep.,1970(54):708-712.
[2] Ullstrup A J.The impacts of the southern corn leaf blight epidemics of 1970-1971[J].Ann Rev Phytopathol,1972,10:37-50.
[3] Smith D R,Hooker A L,Lim S M.Physiologic races of Helminthosporium maydis[J].Plant Dis Rep,1970,54:819-822.
[4] Miller R J,Koeppe D E.Southern corn leaf blight:Susceptible and resistant mitochondria[J].Science,1971,173:67-69.
[5] Bhullar B S,Daly J M,Rehfeld D W.Inhibition of dark CO2 fixation and photosynthesis in leaf discs of corn susceptible to the host-specific toxin produced by Helminthosporium maydis race T[J].Plant Physiol,1975,56:1-7.
[6] Wei J K,Liu K M,Chen J P,et al.Pathological and physiological identification of race C of Bipolaris maydis in China[J].The American Phytopathological Society,1988,78:550-554.
[7] Wei J K,Liu K M,Chen J P,et al.Pathological and physiological identification of race C of Bipolaris maydis in China[J].Phytopathology,1988,78(5):550-554.
[8] 郭兰凯,刘俊芳,马春红,等.HMC毒素对雄花不育及正常细胞质玉米根冠活细胞的影响[J].作物学报,1991,17(1):54-57.
[9] 崔洋,刘克明,魏建昆,等.玉米小班病菌C小种毒素的分离、纯化及其植物病理反应[J].植物病理学报,1991,21(3):187-191
[10] 崔洋,马春红,刘克明,等.纯化的玉米小班病菌C小种毒素生物活性的研究[J].植物病理学报,1992,22(2):175-178.
[11] 刘克明,吴全安,刘俊芳,等.玉米小班病菌三个生理小种生物学特性比较的初步研究[J].华北农学报,1989,4(2):74-78.
[12] 崔洋,刘克明,魏建昆.用可溶性蛋白电泳法鉴定玉米小班病菌生理小种[J].华北农学报,1991,6(1):48-52.
[13] Nicholson P,Rezanoor H N,Su H.Use of randon amplified polymorphic DNA (RAPD) analysis and genetic fingerprinting to differentiate isolate of race O,C and T of Bipolaris maydis[J].J Phytopathology,1993,139:261-267.
[14] Allen S.J.Genetic and induced resistance strategies for controlling Fusarium wilt of cotton[C]//In Proceedings of the Second Australasian Soilborn Diseases symposium,Victoria,Australia,2001:59-60.
[15] Kessmann H,Staub T,Hofmann C,et al.Induction of systemic acquired disease resistance in plants by chemicals[J].Annual Review of Phytopathology,1994,32:439-459.
[16] 张元恩.植物诱导抗性研究进展[J].生物防治通报,1987,3(2):88－90.
[17] 李冠,欧阳光察.植物诱导抗病性[J].植物生理学通讯,1990(6):1－5.
[18] 海蒂弗斯.植物病理生理学[M].朱有红,宋左衡.北京:中国农业出版社,1991:27.
[19] 周仲铭.林木病理学[M].北京:中国林业出版社,1987,84.
[20] 马春红,李运朝,董文琦,等.低浓度玉米小斑病菌T 毒素对玉米叶片苯丙氨酸解氨酶活性的诱导研究[J].华北农学报,2007,22(1):40-43.
[21] Ma Chun-hong,Zhai Cai-xia,Wang Li-an,et al.Induced resistance by the toxin filtrate of Bipolaris maydis race T cultivation[J].Agricultural Sciences in China,2006,5(9):678-684.
[22] 王立安,赵俊霞,马春红,等.玉米根冠脱落细胞中微丝分布的荧光显微观察[J].实验生物学报,2003,36(2):149－154.
[23] 王立安,马春红,赵俊霞,等.HMC毒素对C型不育玉米根冠细胞原生质体及微丝分布的影响[J].植物病理学报,2003,33(3):225－228.
[24] 闫芝芬,马春红,魏建昆.稻瘟病菌致病毒素对水稻雄性不育细胞质的专化性致病机理[J].中国农业科学,1998,31(6):56－61.
[25] 郭红莲,陈婕,高增贵,等.不同诱抗剂诱导玉米对灰斑病的抗性及其与PAL的关系[J].沈阳农业大学学报,2000,31(5):465－467.
[26] 汤章城.现代植物生理学实验指南[M].北京:科学出版社,1999:305.

Please choose a citation manager

Content to export