Abstract: The medium and condition of liquid fermentation for biomass production of Bacillus subtilis B0₃ were optimized with mono-factor experiment and uniform design.The most suitable medium for living cell production was formulated with the ingredients:3.0% corn meal,6.0% soybean oil meal and 0.3% K2HPO4·3H₂O.The optimized fermentation process was as follows:the seed culture was cultured for 21-24 h,the pH of production medium was adjusted to 6.0 before sterilization,the fermentation was carried out in 500 mL erlenmeyer flask with 50 mL production medium inoculated with 5% seed culture and incubated at 35℃,180 r/min for 60-66 h.The biomass density of 104.24×108-105.75×108 cfu/mL was obtained with optimized formula,which was over 96% higher than that produced by basic medium and initial fermentation process.

Key words: Bacillus subtilis, Biomass production, Liquid fermentation, Uniform design

CLC Number: 

• TQ920.6

• S482.28