Construction of Recombinant Marek’s Disease Virus Expressing Avian Influenza Virus HA Gene

doi:10.7668/hbnxb.2007.04.039

ACTA AGRICULTURAE BOREALI-SINICA ›› 2007, Vol. 22 ›› Issue (4): 168-171. doi: 10.7668/hbnxb.2007.04.039

Special Issue: Biotechnology；

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Construction of Recombinant Marek’s Disease Virus Expressing Avian Influenza Virus HA Gene

ZHOU Xue-mei^1,2, HUO Hui-ling², SHE Rui-ping¹, LI Yong-qing³, FENG Guo-min⁴, ZHANG Zhen-hua³, WANG Hong-jun³

1. Department of Veterinary Medicine, China Agricultural University, Beijing 100094, China;
2. Hebei Province Institute of Veterinary Drug Control, Shijiazhuang 050051, China;
3. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089, China;
4. Department of Veterinary Medicine, Jiangxi Agricultural University, Nanchang 330045, China

Received:2007-04-12 Published:2007-08-28

Abstract

Abstract: In this study, a 1.7 kb DNA fragment encoding the HA gene of Avian influenza virus H9N2 was obtained by RT-PCR and the sequence was cloned into the vector of pcDNA; Then the expression cassette including the HA gene of AIV and LacZ gene of Ecoli controlled by CMV promoter, were inserted into US2 gene of MDV to give a transfer vector. The complex of pUS2 HA and DOTAP was transfected into CEF infected MDV. The recombinant MDV were selected and purified by blue plague. The complete HA gene inserted into MDV was detected by PCR. Western-blot and immunostaining result demonstrated tHAt HA protein of AIV was expressed by recombinant MDV.

Key words: Avian influenza virus, HA gene, Recombinant marek’s disease virus

CLC Number:

S852.65

ZHOU Xue-mei, HUO Hui-ling, SHE Rui-ping, LI Yong-qing, FENG Guo-min, ZHANG Zhen-hua, WANG Hong-jun. Construction of Recombinant Marek’s Disease Virus Expressing Avian Influenza Virus HA Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 168-171. doi: 10.7668/hbnxb.2007.04.039.

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References

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