Evaluation and Validation of Eight Molecular Markers Associated with Rice Stripe Resistance in Rice ( Oryza sativa)

doi:10.3969/j.issn.1000-7091.2012.06.029

Abstract

Abstract: In this study，we developed eight resistance-associated markers ST-10，H11-8，H11-12，M68． 4， M79． 1，Rm₂1-8，Rm₂1-15 and H21，as reported previously， to evaluate the resistance of RSD in 24 rice varieties ( including two anti-and sense-varieties) and two F2 populations obtained from a cross between different anti-and sense-varieties． To breed new RSD-resistant rice varieties，we also compared the effectiveness and selection efficiency of these eight markers，and screened the most efficient markers to assist breeding． Qur results showed that: only the consistency rate of markers ST-10 and H11-8 between molecular detection and field phenotypic identification was greater than or close to 90%; The rest of the molecular markers were lower than 80%; Of these， three SSR markers were almost no polymorphism． Compared to STS marker H11-8，SCAR marker ST-10 was more effective for screening RSD resistant varieties． The combined use of these two markers was more accurate and efficient to detect the Stv-bi gene and to further improve the selection efficiency．

Key words: Rice, Rice stripe disease, Resistance gene, Molecular detection, SCAR, Indel, STS, SSR

CLC Number:

S435

YAO Shu, ZHANG Ya-dong, ZHU Zhen, CHEN Tao, ZHAO Qing-yong, ZHOU Li-hui, YU Xin, WANG Cai-lin. Evaluation and Validation of Eight Molecular Markers Associated with Rice Stripe Resistance in Rice ( Oryza sativa)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 146-151. doi: 10.3969/j.issn.1000-7091.2012.06.029.

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References

[1] 白雪亮，王金菊，周维，等．水稻条纹叶枯病的研究进展[J]．生物学通报， 2007， 42( 8) : 4-6．
[2] 王宝祥．灰飞虱传播的水稻黑条矮缩病和条纹叶枯病抗性QTL 定位[D]．南京: 南京农业大学， 2010．
[3] 王才林．江苏省水稻条纹叶枯病抗性育种研究进展[J]．江苏农业科学，2006( 3) : 1-5．
[4] 王才林，张亚东，朱镇，等．水稻条纹叶枯病抗性育种研究[J]．作物学报， 2008，4( 3) : 530-533．
[5] Washio O，Ezuka A，Toriyama K，et al． Testing method for， genetics of and breeding for resistance to rice stripe disease[R]． Bull Chugoku Agr Exp Station，1968，16: 39-197．
[6] Hayano-Saito Y，Tsuji T，Fujii K， et al． Localization of the rice stripe disease resistance gene Stv-bi，by graphical genotyping and linkage analyses with molecular，markers[J]． Theor Appl Genet， 1998， 96: 1044-1049．
[7] Hayano-Saito Y，Saito K，Nakamura S， et al． Fine physical mapping of the rice stripe resistance gene locus Stv-bi[J]． Theor Appl Genet， 2000， 101: 59-63．
[8] Tsuji N． SCAR marker for selection of the rice stripe resistance gene Stv-bi[J]． Japan J Breed Res，2000 ( 2) : 67-72．
[9] 陈峰，张士永，朱文银，等．分子标记辅助选择改良圣稻13 和圣稻14 的条纹叶枯病抗性[J]．中国农业科学， 2010， 43( 16) : 3271-3279．
[10] 潘学彪，陈宗祥，左示敏，等．以分子标记辅助选择育成抗条纹叶枯病水稻新品种“武陵粳1 号”[J]．作物学报， 2009， 35( 10) : 1851-1857．
[11] 李余生，陈涛，虞秋成，等．水稻抗条纹叶枯病基因Stv-bi连锁分子标记的鉴定及利用[J]．江苏农业学报， 2009， 25( 3) : 459-463．
[12] 姚姝，陈涛，张亚东，等．江苏省部分粳稻品种对条纹叶枯病的抗性鉴定及分子检测[J]．江苏农业学报， 2009， 25( 6) : 1201-1206．
[13] Washio O，Ezuka A，Sakurgi Y，et al． Studies on the breeding of rice varieties resistant to stripe diseaseⅠ． Varietal difference in resistence to rice stripe disease[J]． Japan J Breeding， 1976， 17( 1) : 91-98．
[14] 周彤，周益军，程兆榜，等．粳稻品种对水稻条纹叶枯病的抗性鉴定及抗病品种镇稻88 的遗传分析[J]．植物保护学报， 2007， 34( 5) : 475-479．
[15] Dellapporta S L，Wood J，Hicks J B． A plant DNA mini preparation: VersionⅡ[J]． Plant Mol Biol Rep， 1983，1 : 19-21．
[16] Chen F，Temnykh S，Xu Y， et al． Development of a microsatellite framework map providing genome wide coverage in rice ( Oryza sativaL．) [J]． Theor Appl Genet， 1997， 95: 553-567．
[17] 刘志文，傅廷栋，刘雪平，等．作物分子标记辅助选择的研究进展、影响因素及其发展策略[J]．植物学通报， 2005， 22( 增刊) : 82-90．
[18] 郑康乐，黄宁．标记辅助选择在水稻改良中的应用前景[J]．遗传， 1997， 19( 2) : 40．
[19] Wu S J，Zhong H，Zhou Y， et al． Identification of QTLs for the resistance to rice stripe virus in the indica rice variety Dular[J]． Euphytica， 2009， 165: 557-560．
[20] Fuentes J L，Correa-Victoria F J，Escobar F， et al． Identification of microsatellite markers linked to the blast resistance gene Pi-1( t) in rice[J]． Euphytica， 2008， 160: 295-304．

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