Effect of PEG-6000 Priming on Seed Membrane Permeability and the Activity of Protection Enzyme of Aging Soybean

doi:10.3969/j.issn.1000-7091.2012.06.023

Abstract

Abstract: After the seeds of 3 soybean varieties，Heizhenzhu， Jindou 19， Jinda 74 were artificially aged under the air tight condition of high temperature( ( 48 ± 1) ℃) and high humidity( 100% relative humidity), then primed by 10%， 20% and 30%PEG-6000，variation of membrane permeability and the activities of protection enzymes were tested． The results showed that relative electrical conductivity of treated seeds decreased and then increased at first and subsequently decreased and rose again with extension of aging time; MDA content decreased continuously． POD activity declined at first slowly， then increased， finally declined rapidly; SOD activity rose slowly at first and then rapidly declined． After seeds aged of 3 soybean varieties were primed with different concentration PEG-6000， the membrane permeability and MDA conent reduced relatively，and the activities of protection enzymes raised． It suggested that seed resistance to abiotic stress was enhanced． In particular，after the seeds with aging slightly were primed by 20% and 30% PEG-6000，abilities of resistance to aging were significantly enhanced．

Key words: Soybean, Aging, PEG-6000 priming, Conductivity, Protection enzyme

CLC Number:

S565.01

WANG Shu-guang, ZHAO Jian-kui, NING Xing-lian, SHI Yu-gang, SUN Dai-zhen. Effect of PEG-6000 Priming on Seed Membrane Permeability and the Activity of Protection Enzyme of Aging Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 113-117. doi: 10.3969/j.issn.1000-7091.2012.06.023.

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References

[1] 麻浩，孙庆泉．种子加工与贮藏[M]．北京: 中国农业出版社， 2007: 362-366．
[2] 卢新雄，曹永生．作物种质资源保存现状与展望[J]．中国农业科技导报， 2001( 3) : 43-47．
[3] Zheng G H， Jing X M． Ultradry seed storage cuts cost of gene bank[J]． Nature， 1998， 393: 223-224．
[4] 顾龚平，吴国荣，陆长梅，等． PEG 处理对大豆幼苗活力及活性氧代谢的影响[J]．中国油料作物学报，2000， 22( 2) : 26-30．
[5] 王炜，史雨刚，王曙光． PEG 引发对老化大豆种子发芽及活力的影响[J]．山西农业科学， 2011， 39( 7) : 650-654．
[6] 韩玉虎，王勇，程慧，等．金薄香核桃枝条电导率的测定[J]．华北农学报， 2007， 22( 1) : 56-58．
[7] 中国科学院上海植物生理研究所．现代植物生理学实验指南[M]．北京: 科学出版社， 1999: 305．
[8] 杨鹏辉，李贵全，郭丽，等．干旱胁迫对不同抗旱大豆品种质膜透性的影响[J]．山西农业科学，2003，31( 3) : 23-26．
[9] 史雨刚，马金虎，杨进文，等．高温处理对4 个大豆品种种子发芽及活力的影响[J]．山西农业大学学报: 自然科学版， 2008， 28( 4) : 409-412．
[10] 乔燕祥，高平平，马俊华，等．两个玉米自交系在种子老化过程中的生理特性和种子活力变化的研究[J]．作物学报， 2003( 1) : 123-127．
[11] 朱俊刚，王曙光，李晓燕，等． PEG 胁迫对六倍体小黑麦幼苗SOD POD 活性及MDA 含量的影响[J]．中国农学通报， 2009， 25( 18) : 202-204．
[12] 李柏林，梅慧生．燕麦叶片衰老与活性氧代谢的关系[J]．植物生理学报， 1989， 15( 1) : 6-12．
[13] 马文丽，王转花．铅胁迫对乌麦及普通小麦抗氧化酶的影响[J]．山西农业科学， 2004， 32( 2) : 8-12．
[14] 牛远，梁建萍，张建达，等．高温胁迫对华北落叶松幼苗抗氧化酶的影响[J]．山西农业科学，2008，36( 10) : 47-49．
[15] 李明，王根轩．干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响[J]．生态学报， 2002， 22( 4) :503-507．
[16] 赵丽英，邓西平，山仑．活性氧清除系统对干旱胁迫的响应机制[J]．西北植物学报， 2005，25( 2) : 413-418．

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