Mapping QTL for Several Drought Related Traits in Maize(Zea mays L.) under Field Condition

doi:10.3969/j.issn.1000-7091.2012.01.015

Abstract

Abstract: Drought is a major cause of yield loss in maize production all over the world. The objective of this study was to understand the genetic causes underlying drought resistance and to identify relevant quantitative trait locus(QTL). a F_2:3 mapping populations with 160 families derived from crosses of Lin 1(drought resistance) and Xiang97-7(drought sensitive) were phenotyped under the well watered and water stressed treatments in two environments including Gaoqiao and Crop institute of Chang sha in 2011. For the four target traits(anthesis to silking interval, plant height, 1000 kernel weight and grain yield per plant) were measured and the drought resistance index(DRI) for each trait was considered as measurements for drought resistance. The result indicated that a linkage map was constructed based on 110 polymorphic simple sequence repeat(SSR), resulting in a length of 1246. 1 cM across maize genome, with an average inter-marker distance of 11. 33 cM. A total of 43 QTL were identified, with a minimum of 8 and a maximum of 14 for drought related traits. Phenotypic variation associated with each QTL ranges from 6. 27% to 18. 27%. Very few QTL were detected in two water treatments, indicating the different mechanisms may indeed coexist that together contribute to adaptation to drought stress. The QTL for drought related traits appeared to be dispersed across 10 different chromosomes, except chromosomes 2 and 10. Most QTL for drought related traits in this study tended to cluster in some areas of the chromosome 1 and 3, and mainly in bin 1. 02-03, 1. 06-07 and 3. 04-05. Two QTL clusters on chromosome 1 flanked by the markers umc2224 and bnlg176 for plant height, 1000 kernel weight and grain yield per plant, a nd bnlg1556 and umc1128 for anthesis to silking interval and grain yield per plant were identified. A cluster of QTL flanked by the markers umc1773 and umc1311 for plant height, 1000 kernel weight and grain yield per plant were identified on chromosome 3. These novel QTL clusters generated in this study may be used to enhance maize drought resistance by marker assisted selection(MAS) in water limited environments.

Key words: Drought stress, Drought resistance index(DRI), Simple sequence repeat(SSR), Marker assisted selection(MAS)

CLC Number:

S513.03

CHEN Zhi-hui, CAO Zhong-yang, TANG Bin, LI Li. Mapping QTL for Several Drought Related Traits in Maize(Zea mays L.) under Field Condition[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(1): 79-86. doi: 10.3969/j.issn.1000-7091.2012.01.015.

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