Comparison of Two Maize SSR Detection Methods: Capillary Electrophoresis with Fluorescence Detection Method and Denaturing PAGE Silver-staining Detection Method

doi:10.3321/j.issn:1000-7091.2006.05.016

Abstract

Abstract: Seven SSR primers and 192 maize hybrid were used to comparatively analyze SSR markers between capillary electrophoresis with fluorescence detection and denaturing PAGE(polyacrylamide gel electrophoresis) silver-staining detection.The results indicated that SSR markers fragment sizes explored by two decetion systems were relatively similar.Capillary electrophoresis with fluorescence detection has the advantages of high accuracy,sensitiveness,cost effectiveness and high-throught.Therefore,the capillary electrophoresis with fluorescence detection system is suggested to be suitable for scaning and analyzing large-scale material.While denaturing PAGE silver-staining detection would be used in examaining a few samples,especially in screening SSR markers.

Key words: Maize, SSR, Capillary electrophoresis, Silver_staining

CLC Number:

S513

YI Hong-mei, WANG Feng-ge, ZHAO Jiu-ran, WANG Lu, GUO Jing-lun, YUAN Ya-ping. Comparison of Two Maize SSR Detection Methods: Capillary Electrophoresis with Fluorescence Detection Method and Denaturing PAGE Silver-staining Detection Method[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(5): 64-67. doi: 10.3321/j.issn:1000-7091.2006.05.016.

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