Several Improvements for Bioassay of Verticillium dahliae Kleb Coarse Toxin in Cotton Root Cap Cell

doi:10.3321/j.issn:1000-7091.2006.01.013

Abstract

Abstract: Several problems for attention to the bioassay of cotton root cap cell with Verticillium dahliae Kleb coarse toxin were studied.The text compared with the old way:The shaking time for collecting the root cap cell should be controlled in 1-2 min;Root cap cell in the culture chamber with darkness was taken 2-6 h;The natural red(pH=6.5,0.01% for 3~5 min) and Evans blue(0.2% for 3min) as dye stuff were used.The results indicated that with analyzing living cell of root cap treated by Verticillium dahliae Kleb toxin was a quick way in the room to identify the disease resistance of different cotton varieties.

Key words: Cotton, Verticillium dahliae Kleb, Toxin, Root cap cells, Biological test, Pigmentation

CLC Number:

S562.01

FAN Wei-wei, CHEN Xia, MA Chun-hong, LI Guang-min, ZHANG Xiang-yun, GENG Jun-yi. Several Improvements for Bioassay of Verticillium dahliae Kleb Coarse Toxin in Cotton Root Cap Cell[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(1): 55-58. doi: 10.3321/j.issn:1000-7091.2006.01.013.

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