Polymorphism Analysis by Sequence-related Amplified Polymorphism Markers in Brassica napus L

doi:10.3321/j.issn:1000-7091.2006.01.012

Abstract

Abstract: Polymorphism among 1141B,KenC₁,32B and 32C were analyzed with SRAP(sequence-related amplified polymorphism) markers.There were 13-36 clear polymorphic combination bands per primer,and the average was 5.8.About 419 amplified fragments and 116 polymorphic fragments were detected 20 SRAP primer combinations.The percentage of polymorphic fragments was 28.23%.The number of amplified fragments and polymorphic fragments per primer combination were 20.95 and 5.8,respectively.The polymorphism analyzed by F₂ individual of 1141B Ken C₁ with 24 primer combinations showed that there were 75 polymorphic bands,the average was 3.12.The χ² test for allelic genotype of all loci indicated that 1.33%of the SRAP markers distorted from the expectation 3:1 ratio(dominance markers) and 1:2:1(co-dominance markers).It seems that SRAP markers was a high efficiency marker system in the paper.It showed that SRAP were an effective,cheap and dependable marker for polymorphism analysis and linkage map construction in B.napus L.

Key words: B.napus L., Polymorphim, SRAP marker

CLC Number:

S565

ZHANG Shu-fen, FU Ting-dong, LI Yuan-yuan, ZHU Jia-cheng, WANG Jian-ping, MA Chao-zhi. Polymorphism Analysis by Sequence-related Amplified Polymorphism Markers in Brassica napus L[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(1): 50-54. doi: 10.3321/j.issn:1000-7091.2006.01.012.

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References

[1] 方宣钧,吴为人,唐纪良.作物DNA标记辅助育种[M].北京:科学出版社,2002:1-9.
[2] 马朝芝,傅廷栋,Stine Tuevesson,et al.用ISSR标记技术分析中国和瑞典甘蓝型油菜的遗传多样性[J].中国农业科学2003,36(11):1403-1408.
[3] Li G,Quiros C F.Sequence-related amplified polymorphism(SRAP),a new marker system based on a simple PCR reaction:its application to maping and gene tagging in brassica[J].Theor Appl Genet,2001,103:455-461.
[4] Li G,Gao M,Yang B,et al.Gene for gene alignment between the brassica and Arabidopsis genomes by direct transcriptome mapping[J].Theor Appl Genet,2003,107:168-180.
[5] Riaz Ahmad,Dan Potter,Stephen M Southwick.Genotyping of peach and nectarine cultivars with SSR and SRAP moleullar marker[J].J Amer Soc Hort Soi,2004,129(2):204-210.
[6] Budak H,Shearman R C,Parmaksiz I,et al.Molecular characterization of Buffalograss germplasm using sequencerelated amplified polymorphism markers [J].Theor Appl Genet,2004,108:328-334.
[7] Budak H,Shearman R C,Gaussoin R E,et al.Application of sequence-related amplified polymorphism markers for characterization of turfgrass species[J].Hortscience,2004,39(5):955-958.
[8] Ferriol M,Pico B,Nuez F.Genetic diversity of a germplasm collection of cucurbita pepo using SRAP and AFLP markers[J].Theor Appl Genet,2003,107:271-282.
[9] Budak H,Shearman R C,Parmaksiz I,et al.Comparative analysis of seeded and vegetative biotype buffalograsses based on phylogenetic relationship using ISSRs,SSRs,RAPDs and SRAPs[J].Theor Appl Genet,2004,109:280-288.
[10] 林忠旭,张献龙,聂以春,等.棉花SRAP遗传连锁图构建[J].科学通报,2003,48(15):1676-1679.
[11] 李佳,沈斌章,韩继祥.一种有效提取油菜叶片总DNA的方法[J].华中农业大学学报,1994,13(5):51-62.
[12] 陆光远,杨光圣,傅廷栋.应用于油菜研究的简便银染AFLP标记技术的构建[J].华中农业大学学报,2001,20(5):413-415.
[13] Garcia A F,Benchimol L L,Barbosa A M,et al.Comparison of RAPD,RFLP,AFLP and SSR markers for diversity studies in tropical maize inbred lines [J].Theor Appl Genet,2004,27(4):579-588.
[14] 高玉千,张书芬,朱家成,等.应用AFLP标记筛选甘蓝型油菜R4和22B的多态性[J].河南农业科学2005,(8):32-35.
[15] Lyttle T W.Segregation distorters[J].Annu Rev Genet,1991,25(1):511-557.
[16] Kinshita T.Report of the committee on gene symbolization,nomenclature and linkage group [J].Rice Genet Newl,1993,101:7-39.
[17] Coe H E,Polacco M.Gene list and working maps[J].Maize Genet Coop Newslett,1995,69 (1):157-191.
[18] Knox M R,Ellis T H.Excess heterozygosity contributes to genetic map expansion in pea recombinant inbred populations[J].Genetics,2002,162 (2):861-873.
[19] 陆光远,杨光圣,傅廷栋.甘蓝型油菜分子标记连锁图谱的构建及显性细胞核雄性不育基因的图谱定位[J].遗传学报,2004,31(11):1309-1315.
[20] Zunova M U,Ecke W,Weissleder K,et al.Mapping the genome of rapeseed (Brassica napus L) I.Construction of an RFLP linkage map and localization of QTLs for seed glucosinolate content [J].Theor Appl Genet,1995,90 (2):194-204.

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