Study on the Transformation of Cotton Mediated with Agrobacterium tumefacines

doi:10.3321/j.issn:1000-7091.2002.z1.017

Abstract

Abstract: Hypocotyl used as explants,twenty varieties or lines were studied for plant regeneration or somatic embryoid.Nineteen of them were successful.According to the result,the varieties easy in plant regeneration Han 93-2,Hanwu 2031,Hengwu 89-30,Coker 201,Coker 312 were chosen for the transformation.Inoculating rate of Kanamycine resistant callus was about 50%,among them 40% was GUS positive.50% of plants regenerated from the GUS positive callus were GUS positive,and proved by Southern hybridization analysis.

Key words: Agrobacterium tumefacine, Cotton, Gene transformation

CLC Number:

S562.035

LI Jun-lan, ZHANG Han-shuang, GAO Peng, WANG Hai-bo. Study on the Transformation of Cotton Mediated with Agrobacterium tumefacines[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(S1): 80-84. doi: 10.3321/j.issn:1000-7091.2002.z1.017.

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