Study on in vitro Screening of Resistant to Blast Using Crude-toxin in Rice

doi:10.3321/j.issn:1000-7091.2002.03.019

Abstract

Abstract: Rice callus of eembryo-bud and pollen were screened for blast-resistance in vitro under selection pressure of crude toxin.The result indicated that the crude toxin evidently inhibit the callus from eembryo-bud and pollen cultured,dif ferentiation in vitro.As the increasing of the crude toxin concentration,the inhibiting getting enhanced.Toxicity of sterilization filtration method was higher than high pressure steam filtration method.Inhibiting of pollen higher than that of eembryo-bud in crude toxin,and crude toxin concentration for screen pollen strains with resistance to blast was lower than that of screen somatic cell strains with resistance of blast.Crude toxin can be used selection pressure for selection of rice plant with resistance blast.

Key words: Rice, Pyricularia oryzae Cav, Crude toxin, in vitro screening

CLC Number:

S435.111.4+.1

PIAO Zhongze, NIU Jing, LI Yanping, ZOU Meizhi, HAN Longzhi. Study on in vitro Screening of Resistant to Blast Using Crude-toxin in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(3): 94-98. doi: 10.3321/j.issn:1000-7091.2002.03.019.

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