萝卜赤霉素生物合成和信号转导相关基因的全基因组鉴定及表达分析

doi:10.7668/hbnxb.20193146

摘要/Abstract

摘要：

赤霉素途径是植物开花调控中的一条重要途径。为了解赤霉素途径相关基因在萝卜开花调控中的作用,利用生物信息学方法对萝卜赤霉素生物合成和信号转导相关基因的结构、理化性质、染色体分布、启动子顺式作用元件和组织特异性表达进行了分析,并对不同开花期萝卜材料中这些基因的表达水平进行了实时荧光定量 PCR检测。结果表明,萝卜基因组含有46个赤霉素生物合成和信号转导相关基因,CPS、KS、KO、KAO、GA20OX、GA3OX、GA2OX、GAI、RGA、RGL、GID1、SKP2分别有2,1,2,2,9,5,12,1,1,4,3,4个,它们不均匀分布于9条染色体上,其编码蛋白质分子质量为21.32~127.80 ku,等电点为4.72~9.04;基因结构和保守结构域分析发现,这46个基因的外显子数为1~21个不等,且一些保守基序为大部分基因所共有。启动子顺式作用元件分析表明,这46个基因的启动子含有与光、赤霉素、生长素、ABA、SA、低温、干旱等响应的顺式作用元件。利用萝卜基因表达数据库分析发现,这46个基因在不同组织和不同发育时期的表达量不同;实时荧光定量PCR检测表明,这些基因在早开花材料心里美萝卜和晚开花材料野萝卜中的表达量有明显差异,暗示其可能与萝卜的生殖生长有较密切的关系。

关键词: 萝卜, 赤霉素生物合成, 信号转导, 基因, 表达分析

Abstract:

Gibberellin pathway is an important pathway in plant flowering regulation.In order to understand the role of gibberellin pathway related genes in the regulation of radish flowering. The structure,physicochemical properties,chromosome distribution,promoter cis-elements and tissue-specific expression of gibberellin biosynthesis and signal transduction related genes in radish were analyzed by bioinformatics.The expression levels of these genes in radish varieties with different florescence were detected by Real-time fluorescence quantitative PCR(qPCR).The results showed there were 46 genes related to gibberellin biosynthesis and signal transduction in radish genome,among them,the gene numbers of CPS,KS,KO,KAO,GA20OX,GA3OX,GA2OX,GAI,RGA,RGL,GID1 and SKP2 were 2,1,2,2,9,5,12,1,1,4,3 and 4,respectively.They were unevenly distributed on 9 chromosomes,molecular weight of their coding proteins were 21.32—127.80 ku,and the isoelectric points of the proteins were from 4.72 to 9.04.The analysis of gene structure and conserved domain showed that the number of exons of these 46 genes ranged from 1 to 21,and some conserved motifs were shared by most genes.Promoter cis-elements analysis showed that the promoters of these 46 genes contained cis-elements related to light,gibberellin,auxin,ABA,SA,low temperature,drought,etc.Using radish gene expression database analysis,it was found that the expression levels of these 46 genes were different in different tissues and at different developmental stages;qPCR detection showed that there were significant differences in the expression of these genes between early flowering material Xinlimei and late flowering material wild radish,suggesting that they may be closely related to the reproductive growth of radish.

Key words: Radish, Gibberellin biosynthesis, Signal transduction, Gene, Expression analysis

阳文龙, 李锡香, 张晓辉, 宋江萍, 贾会霞, 王海平. 萝卜赤霉素生物合成和信号转导相关基因的全基因组鉴定及表达分析[J]. 华北农学报, 2022, 37(6): 1-14. doi: 10.7668/hbnxb.20193146.

YANG Wenlong, LI Xixiang, ZHANG Xiaohui, SONG Jiangping, JIA Huixia, WANG Haiping. Genome-wide Identification and Expression Analysis of Genes Related to Gibberellin Biosynthesis and Signal Transduction in Radish[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(6): 1-14. doi: 10.7668/hbnxb.20193146.

http://www.hbnxb.net/CN/Y2022/V37/I6/1

图/表 10

参考文献 51

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基因 Gene	正向引物序列(5'—3') Forward primer sequence (5'—3')			反向引物序列(5'—3') Reverse primer sequence (5'—3')
RsGAPDH	ACCACTAACTGCCTTGCTCC			AGCTCTTCCACCTCTCCAGT
RsCPS	CCTGCGTCGTTGCTCTAACAT			CTCAAGTAATGAAGGAAATGCCACT
RsKS	CAAGATTGGGATTTGGTTGGT			AGGGATAAACTGTGGGAACTGC
RsKO	TTCCCATTCGCTATGCTCAT			TCATCCAAGAACCTCTCAGGC
RsKAO	CGGTTTGTCATTGGTCACAGT			GAAATCAGCATCCCACATTGAA
RsGA20OX1	CGCTCCATTAGTCCCAACC			GTGAAGTCAGGGTATCTCCTTGCT
RsGA20OX2	GCATAGAGCGGTTGTGAACAG			GCCAAGTGAAATCAGGGTATCTC
RsGA20OX3	TTCCTCCTAACCCTCAATCAGTT			CTCTCTTTCACCAGACATCATTCTC
RsGA20OX4	TCTCTAACCATACTTCAACAAGACTACAGT			TCTCACTATTCACTACCGCCTGAT
RsGA3OX1	GCGTCAGCGAAGAAGACATT			CTTGGAGACCAGCAGTGTTGTT
RsGA3OX2	CGTCGGTGACTTACTCCACATT			TGCTTCCAAGAAAGAGATGGG
RsGA3OX3	CCTCACACTGACTCTTCCCTACTG			GAGACAAATCGCCCATTATGAC
RsGA3OX4	TACGGTAGAGCCGATTCCTG			GGACTTATCTCCACATCACCAGC
基因 Gene		正向引物序列(5'—3') Forward primer sequence (5'—3')	反向引物序列(5'—3') Reverse primer sequence (5'—3')
RsGAI		GTGTTGGGCTACAAAGTCAGG	GGGTTGAACTCCGTGAGC
RsRGA		GCTCATCTCGCCACGGAT	ACGCTTGTTGTATTGATTCGGA
RsGID1-1		GTTTCCGAAGAGTCTCGTGG	ACGCAGCAATCTCATCCAT
RsGID1-2		CTTGTCGTTGTTGCTGGTTTAGAT	GTGCTTCGGCTACACTCCTCT
RsGID1-3		GACCGTGATTGGTATTGGAGA	CCGCACTCTTGAGCCCTT
RsFLC		ATCCGACATCAATCTTCCG	TACACACACAATCTCTCAGCCA
RsFRI		ATACCTTTGGGATTGAGGATAGG	TCTCTGTGTCTAAGTTGTGAGCCT
RsFT		TGCGGAGGACGAAGAAGTT	TATTATCACCGTTCATTGCTCG

基因 Gene	正向引物序列(5'—3') Forward primer sequence (5'—3')			反向引物序列(5'—3') Reverse primer sequence (5'—3')
RsGAPDH	ACCACTAACTGCCTTGCTCC			AGCTCTTCCACCTCTCCAGT
RsCPS	CCTGCGTCGTTGCTCTAACAT			CTCAAGTAATGAAGGAAATGCCACT
RsKS	CAAGATTGGGATTTGGTTGGT			AGGGATAAACTGTGGGAACTGC
RsKO	TTCCCATTCGCTATGCTCAT			TCATCCAAGAACCTCTCAGGC
RsKAO	CGGTTTGTCATTGGTCACAGT			GAAATCAGCATCCCACATTGAA
RsGA20OX1	CGCTCCATTAGTCCCAACC			GTGAAGTCAGGGTATCTCCTTGCT
RsGA20OX2	GCATAGAGCGGTTGTGAACAG			GCCAAGTGAAATCAGGGTATCTC
RsGA20OX3	TTCCTCCTAACCCTCAATCAGTT			CTCTCTTTCACCAGACATCATTCTC
RsGA20OX4	TCTCTAACCATACTTCAACAAGACTACAGT			TCTCACTATTCACTACCGCCTGAT
RsGA3OX1	GCGTCAGCGAAGAAGACATT			CTTGGAGACCAGCAGTGTTGTT
RsGA3OX2	CGTCGGTGACTTACTCCACATT			TGCTTCCAAGAAAGAGATGGG
RsGA3OX3	CCTCACACTGACTCTTCCCTACTG			GAGACAAATCGCCCATTATGAC
RsGA3OX4	TACGGTAGAGCCGATTCCTG			GGACTTATCTCCACATCACCAGC
基因 Gene		正向引物序列(5'—3') Forward primer sequence (5'—3')	反向引物序列(5'—3') Reverse primer sequence (5'—3')
RsGAI		GTGTTGGGCTACAAAGTCAGG	GGGTTGAACTCCGTGAGC
RsRGA		GCTCATCTCGCCACGGAT	ACGCTTGTTGTATTGATTCGGA
RsGID1-1		GTTTCCGAAGAGTCTCGTGG	ACGCAGCAATCTCATCCAT
RsGID1-2		CTTGTCGTTGTTGCTGGTTTAGAT	GTGCTTCGGCTACACTCCTCT
RsGID1-3		GACCGTGATTGGTATTGGAGA	CCGCACTCTTGAGCCCTT
RsFLC		ATCCGACATCAATCTTCCG	TACACACACAATCTCTCAGCCA
RsFRI		ATACCTTTGGGATTGAGGATAGG	TCTCTGTGTCTAAGTTGTGAGCCT
RsFT		TGCGGAGGACGAAGAAGTT	TATTATCACCGTTCATTGCTCG

基因名称 Gene name	基因登录号 Gene accession No.	染色体 Chromosome	染色体位置 Chromosome location	链 Strand	编码区 /bp CDS	蛋白质 /aa Protein	分子质量 /ku MW		等电点 pI	不稳定指数 Instability index	脂肪指数 Aliphatic index	亲水性 GRAVY
RsCPS1	Rs468820	R9	22262393~22269809	+	2 451	816	94.25		5.66	44.38	88.68	-0.296
RsCPS2	Rs527850	RUS00633	3085~10083	+	2 430	809	93.64		5.96	44.29	87.52	-0.321
RsKS	Rs448620	R9	467671~474678	-	3 393	1 130	127.80		5.53	45.94	86.68	-0.257
RsKO1	Rs169040	R4	11324717~11326905	+	1 521	506	57.88		6.46	47.65	91.92	-0.274
RsKO2	Rs574920	RUS01337	9122~11305	+	1 539	512	58.50		6.96	47.31	91.99	-0.268
RsKAO1	Rs003450	R1	1829046~1831774	-	1 707	568	65.92		6.46	40.72	84.28	-0.309
RsKAO2	Rs355870	R6	46815059~46817122	+	1 470	489	56.54		9.04	39.29	88.49	-0.273
RsGA20OX1	Rs049910	R2	37179260~37180704	+	1 143	380	43.68		5.90	48.14	73.13	-0.425
RsGA20OX2	Rs092510	R2	10472001~10473401	-	1 140	379	43.24		5.56	43.67	70.74	-0.449
RsGA20OX3	Rs074900	R2	28031441~28032910	-	1 140	379	43.64		5.69	48.49	68.89	-0.495
RsGA20OX4	Rs145650	R3	27582762~27584109	+	1 128	375	42.72		6.57	44.89	72.24	-0.432
RsGA20OX5	Rs601630	R3	27601766~27603107	+	1 122	373	42.50		6.57	43.90	72.63	-0.437
RsGA20OX6	Rs157440	R4	4617142~4618592	+	1 146	381	43.62		5.97	48.02	74.46	-0.427
基因名称 Gene name	基因登录号 Gene accession No.	染色体 Chromosome	染色体位置 Chromosome location	链 Strand	编码区 /bp CDS	蛋白质 /aa Protein	分子质量 /ku MW	等电点 pI		不稳定指数 Instability index	脂肪指数 Aliphatic index	亲水性 GRAVY
RsGA20OX7	Rs473380	R9	12625480~12626895	+	1 143	380	43.32	6.27		42.14	74.39	-0.351
RsGA20OX8	Rs481550	R9	24415089~24416702	+	1 074	357	40.10	5.78		36.14	74.82	-0.368
RsGA20OX9	Rs481540	R9	24421848~24423450	+	1 074	357	40.06	6.13		38.90	75.10	-0.361
RsGA3OX1	Rs012590	R1	6333424~6334859	-	1 080	359	40.17	5.89		44.75	86.94	-0.227
RsGA3OX2	Rs103440	R2	280484~282019	+	1 164	387	43.49	6.65		43.22	90.44	-0.128
RsGA3OX3	Rs103430	R2	282791~284121	+	1 056	351	38.59	7.65		42.55	92.45	-0.083
RsGA3OX4	Rs338890	R6	33491105~33492531	-	1 074	357	40.42	7.81		44.36	86.55	-0.279
RsGA3OX5	Rs427250	R8	14209249~14210367	+	735	244	27.82	6.30		65.58	83.85	-0.278
RsGA2OX1	Rs023230	R1	13531883~13533834	+	1 020	339	38.08	6.27		53.13	83.04	-0.331
RsGA2OX2	Rs001270	R1	770957~773364	+	990	329	36.41	6.26		48.87	78.84	-0.308
RsGA2OX3	Rs570400	R2	1012769~1014452	+	963	320	35.56	6.66		36.74	81.88	-0.245
RsGA2OX4	Rs052360	R2	34947056~34947691	+	558	185	21.64	8.48		39.32	74.27	-0.404
RsGA2OX5	Rs170920	R4	12212866~12214381	+	984	327	36.72	5.45		31.45	86.64	-0.237
RsGA2OX6	Rs161090	R4	6661715~6662376	-	555	184	21.32	8.47		34.76	72.61	-0.398
RsGA2OX7	Rs268140	R5	33513916~33514668	+	648	215	23.66	5.93		42.74	84.28	-0.120
RsGA2OX8	Rs267680	R5	34128058~34130208	+	999	332	37.95	8.87		47.95	73.01	-0.337
RsGA2OX9	Rs357280	R6	47701447~47704379	+	981	326	36.78	6.28		47.44	80.03	-0.342
RsGA2OX10	Rs419290	R8	7392543~7394486	+	1 023	340	38.23	7.63		47.66	86.50	-0.286
RsGA2OX11	Rs430940	R8	14100094~14100849	+	555	184	21.33	8.16		31.52	73.10	-0.424
RsGA2OX12	Rs449910	R9	1082416~1083847	+	762	253	27.97	5.10		33.98	88.89	-0.155
RsGAI	Rs493610	R9	33197399~33199156	+	1 758	585	64.02	5.48		44.67	79.38	-0.276
RsRGA	Rs179530	R4	17734690~17736416	+	1 599	532	58.15	5.57		45.99	81.79	-0.188
RsRGL1	Rs094860	R2	8444132~8445667	-	1 452	483	53.31	5.38		41.44	91.86	-0.193
RsRGL2	Rs220460	R5	916848~918476	-	1 629	542	59.02	4.72		44.83	83.82	-0.224
RsRGL3	Rs399820	R7	3634445~3636031	+	1 587	528	58.01	4.87		48.52	86.14	-0.207
RsRGL4	Rs541920	RUS00210	27721~29301	+	1 581	526	57.81	4.87		48.77	85.74	-0.226
RsGID1-1	Rs221810	R5	1751276~1752658	+	1 059	352	39.41	6.67		43.00	86.65	-0.259
RsGID1-2	Rs218880	R4	49898901~49900165	+	1 083	360	40.45	6.70		43.51	89.25	-0.139
RsGID1-3	Rs170080	R4	11981312~11982923	-	1 032	343	38.23	6.09		46.41	88.05	-0.176
RsSKP2-1	Rs388660	R7	9192014~9193640	-	1 077	358	39.15	6.86		43.79	99.94	0.059
RsSKP2-2	Rs380870	R7	16044907~16046864	+	1 089	362	39.85	7.76		46.10	97.24	-0.004
RsSKP2-3	Rs414970	R8	4640621~4642318	+	1 095	364	40.05	7.06		42.90	99.12	0.067
RsSKP2-4	Rs415000	R8	4664912~4666661	+	1 122	373	41.13	6.77		47.42	97.42	0.024

基因名称 Gene name	基因登录号 Gene accession No.	染色体 Chromosome	染色体位置 Chromosome location	链 Strand	编码区 /bp CDS	蛋白质 /aa Protein	分子质量 /ku MW		等电点 pI	不稳定指数 Instability index	脂肪指数 Aliphatic index	亲水性 GRAVY
RsCPS1	Rs468820	R9	22262393~22269809	+	2 451	816	94.25		5.66	44.38	88.68	-0.296
RsCPS2	Rs527850	RUS00633	3085~10083	+	2 430	809	93.64		5.96	44.29	87.52	-0.321
RsKS	Rs448620	R9	467671~474678	-	3 393	1 130	127.80		5.53	45.94	86.68	-0.257
RsKO1	Rs169040	R4	11324717~11326905	+	1 521	506	57.88		6.46	47.65	91.92	-0.274
RsKO2	Rs574920	RUS01337	9122~11305	+	1 539	512	58.50		6.96	47.31	91.99	-0.268
RsKAO1	Rs003450	R1	1829046~1831774	-	1 707	568	65.92		6.46	40.72	84.28	-0.309
RsKAO2	Rs355870	R6	46815059~46817122	+	1 470	489	56.54		9.04	39.29	88.49	-0.273
RsGA20OX1	Rs049910	R2	37179260~37180704	+	1 143	380	43.68		5.90	48.14	73.13	-0.425
RsGA20OX2	Rs092510	R2	10472001~10473401	-	1 140	379	43.24		5.56	43.67	70.74	-0.449
RsGA20OX3	Rs074900	R2	28031441~28032910	-	1 140	379	43.64		5.69	48.49	68.89	-0.495
RsGA20OX4	Rs145650	R3	27582762~27584109	+	1 128	375	42.72		6.57	44.89	72.24	-0.432
RsGA20OX5	Rs601630	R3	27601766~27603107	+	1 122	373	42.50		6.57	43.90	72.63	-0.437
RsGA20OX6	Rs157440	R4	4617142~4618592	+	1 146	381	43.62		5.97	48.02	74.46	-0.427
基因名称 Gene name	基因登录号 Gene accession No.	染色体 Chromosome	染色体位置 Chromosome location	链 Strand	编码区 /bp CDS	蛋白质 /aa Protein	分子质量 /ku MW	等电点 pI		不稳定指数 Instability index	脂肪指数 Aliphatic index	亲水性 GRAVY
RsGA20OX7	Rs473380	R9	12625480~12626895	+	1 143	380	43.32	6.27		42.14	74.39	-0.351
RsGA20OX8	Rs481550	R9	24415089~24416702	+	1 074	357	40.10	5.78		36.14	74.82	-0.368
RsGA20OX9	Rs481540	R9	24421848~24423450	+	1 074	357	40.06	6.13		38.90	75.10	-0.361
RsGA3OX1	Rs012590	R1	6333424~6334859	-	1 080	359	40.17	5.89		44.75	86.94	-0.227
RsGA3OX2	Rs103440	R2	280484~282019	+	1 164	387	43.49	6.65		43.22	90.44	-0.128
RsGA3OX3	Rs103430	R2	282791~284121	+	1 056	351	38.59	7.65		42.55	92.45	-0.083
RsGA3OX4	Rs338890	R6	33491105~33492531	-	1 074	357	40.42	7.81		44.36	86.55	-0.279
RsGA3OX5	Rs427250	R8	14209249~14210367	+	735	244	27.82	6.30		65.58	83.85	-0.278
RsGA2OX1	Rs023230	R1	13531883~13533834	+	1 020	339	38.08	6.27		53.13	83.04	-0.331
RsGA2OX2	Rs001270	R1	770957~773364	+	990	329	36.41	6.26		48.87	78.84	-0.308
RsGA2OX3	Rs570400	R2	1012769~1014452	+	963	320	35.56	6.66		36.74	81.88	-0.245
RsGA2OX4	Rs052360	R2	34947056~34947691	+	558	185	21.64	8.48		39.32	74.27	-0.404
RsGA2OX5	Rs170920	R4	12212866~12214381	+	984	327	36.72	5.45		31.45	86.64	-0.237
RsGA2OX6	Rs161090	R4	6661715~6662376	-	555	184	21.32	8.47		34.76	72.61	-0.398
RsGA2OX7	Rs268140	R5	33513916~33514668	+	648	215	23.66	5.93		42.74	84.28	-0.120
RsGA2OX8	Rs267680	R5	34128058~34130208	+	999	332	37.95	8.87		47.95	73.01	-0.337
RsGA2OX9	Rs357280	R6	47701447~47704379	+	981	326	36.78	6.28		47.44	80.03	-0.342
RsGA2OX10	Rs419290	R8	7392543~7394486	+	1 023	340	38.23	7.63		47.66	86.50	-0.286
RsGA2OX11	Rs430940	R8	14100094~14100849	+	555	184	21.33	8.16		31.52	73.10	-0.424
RsGA2OX12	Rs449910	R9	1082416~1083847	+	762	253	27.97	5.10		33.98	88.89	-0.155
RsGAI	Rs493610	R9	33197399~33199156	+	1 758	585	64.02	5.48		44.67	79.38	-0.276
RsRGA	Rs179530	R4	17734690~17736416	+	1 599	532	58.15	5.57		45.99	81.79	-0.188
RsRGL1	Rs094860	R2	8444132~8445667	-	1 452	483	53.31	5.38		41.44	91.86	-0.193
RsRGL2	Rs220460	R5	916848~918476	-	1 629	542	59.02	4.72		44.83	83.82	-0.224
RsRGL3	Rs399820	R7	3634445~3636031	+	1 587	528	58.01	4.87		48.52	86.14	-0.207
RsRGL4	Rs541920	RUS00210	27721~29301	+	1 581	526	57.81	4.87		48.77	85.74	-0.226
RsGID1-1	Rs221810	R5	1751276~1752658	+	1 059	352	39.41	6.67		43.00	86.65	-0.259
RsGID1-2	Rs218880	R4	49898901~49900165	+	1 083	360	40.45	6.70		43.51	89.25	-0.139
RsGID1-3	Rs170080	R4	11981312~11982923	-	1 032	343	38.23	6.09		46.41	88.05	-0.176
RsSKP2-1	Rs388660	R7	9192014~9193640	-	1 077	358	39.15	6.86		43.79	99.94	0.059
RsSKP2-2	Rs380870	R7	16044907~16046864	+	1 089	362	39.85	7.76		46.10	97.24	-0.004
RsSKP2-3	Rs414970	R8	4640621~4642318	+	1 095	364	40.05	7.06		42.90	99.12	0.067
RsSKP2-4	Rs415000	R8	4664912~4666661	+	1 122	373	41.13	6.77		47.42	97.42	0.024

基序 Motif	E-值 E-value	长度/bp Length	序列 Sequence
GA合成上游基因编码蛋白基序Motifs of GA up-stream synthesis protein
1	9.10E-39	41	PISLTAYREAKEDTEIGGYYIPAGPEVATWIAGVHLDPKRW
2	9.40E-21	21	VLPFGLGEHLCIGBRLASLGI
3	3.70E-27	29	ERJITTLACVVALTEWNIYELAYHKGLQF
4	3.50E-19	29	YVHRYWTDKGICWARCSHVQDIDDTAMAF
5	3.60E-22	29	DEKGKTLEREEIFDVLLVELVEGAVDTDH
6	1.50E-19	29	SYEIHKGLPLIGALPVQGEEAPHISIGYI
7	8.00E-32	50	EIARGPNIDVPKDSPVLKDJYAKKELKLTRDPDDFHKKIPRCJLNGLLGA
8	3.40E-35	50	DWLIEWGLSKLELHERDHPQEPVNFEAEREHERFGWAKKQVFGKDIFSIH
9	4.30E-30	50	QYGGENDVWIGKTLYRMPYVNNNEYLELAKQDYNNCQALHQLEWDTFKKW
10	3.50E-25	50	GEEGEYFPPGGQGWPFIGGMFNFLRAFKLADPDEFIRKNAKEYGSKGLHK
GA合成下游基因编码蛋白基序Motifs of GA down-stream synthesis protein
1	1.80E-373	29	WVSVPPBPSALVVNIGDTFQALSNGRFKSMEME
2	5.60E-359	29	JGLGPHTDPTILTILHQBNVGGLQVFVDBMEME
3	6.00E-409	50	AKRLIVEACEKHGFFLVVNHGVPEELJSRAEHEMVSFFDLPASEKLKAARMEME
4	1.90E-209	21	FEDNDSILRLNHYPPCPEPDLMEME
5	8.90E-218	25	HRAVVNSESERKSMAFFLCPKLDKVMEME
6	4.30E-228	41	GQEYEDFGKVVZEYCEAMNKLSLKIMELLGESLGIERRDFRMEME
7	1.30E-115	15	LYPDFTWSEYLEFTQMEME
8	3.10E-154	29	PGESSGYASSFIGRFSTKLPWKETLSFKFMEME
9	6.40E-81	31	IPQQFIWPDHEKPSSEVPELQVPLIDLAGFLMEME
10	2.10E-67	29	EPFGYGSKRIGPNGDVGWIEYJLLNANPQMEME
GA信号转导基因编码蛋白基序Motifs of GA signaling protein
1	1.80E-221	50	EEGLQMHFYESCPYLKFAHFTANQAILEALHTASGVHVJDMSLCQGLQWPMEME
2	4.90E-135	49	PPSQDRVMSEVYLGRQILNLVAAEGADRVERHETLSQWRNRFGSAGFDPMEME
3	6.00E-146	41	VKSLKPKIVTVVEQEARHBGPVFLDRFVEAJHYYSSLFDSLMEME
4	3.00E-112	41	KQASMLLALFGGGDGYRVEENEGCLMLGWQTRPLIATSAWKMEME
5	2.00E-97	50	DLCGCVLITDESVVALADWCVHLRSLGLYYCRNITDRAMYSLAQSGVKNKMEME
6	4.20E-89	50	LNLCGCVEAVSDNALZAIGENCNQMQSLNLGWCEKISDDGVMSLAYGCPDMEME
7	2.20E-86	50	WKDIPVELLMRILNLVDDRTVIIASGVCSGWRDAISFGLTRLRLSWCKKNMEME
8	1.40E-75	21	SAMRKVADYFAEALARRIYRLMEME
9	9.60E-76	50	SDGLQELGWKLAQLAQAIGVEFEFKGLVAESLTDLEPEMFETRPESETLVMEME
10	7.60E-73	32	NMDELLAVLGYKVRSSDMADVAQKLEQLEVVLMEME

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