摘要： 利用28对SSR核心引物构建了25个鲁棉研系列抗虫棉品种的分子指纹图谱并进行遗传多样性分析。28对核心引物的SSR位点分属21条染色体,等位基因数为2~8个,平均4.39个；PIC值为0.63~0.93,平均0.84。数字指纹图谱显示,最少采用BNL1421、BNL1513、BNL1231、NAU2026和BNL2646这5对引物组合可以将25份供试材料区分开。对其中14个常规品种的遗传多样性分析表明,其遗传相似系数介于0.35~0.87,平均为0.57。此结果可为现阶段鲁棉研系列种质资源的杂交选育提供依据。

关键词: 鲁棉研系列品种, 核心引物, SSR指纹图谱, 遗传多样性

Abstract: SSR markers were used to analyze the fingerprinting and genetic diversity of 25 SCRC series transgenic Bt cotton varieties. Twenty-eight primers screened out from 412 SSR primers, with high polymorphisms and good repeatability, were regarded as core primers in the study. These primers whose SSR loci distributed on 21 chromosomes of cotton,have 2-8 alleles, with an average of 4.39; polymorphic information content (PIC) about 0. 63-0.93, with an average of 0. 84. These results suggest the core primers have preferable polymorphism. The digital fingerprintmg was used to indicated all estimate the varietres genetrc could be distinguished by 5 primer combinations at least. UPGMA cluster analysis distances and to construct a dendrogram by NTSYS 2. 1.The similarity coefficients of 14 SCRC conventional varieties were dance of genetic diversity. These results sources. between 0. 35-0. 87,with an average of 0.57,indicating relative abundance of genetic diversity. These results can provide technical support for cross breeding of SCRC germplasm resources.

Key words: SCRC serles varieties, Core primers, SSR fingerprinting, Genetic diversity

中图分类号: 

• S562.03