RNA干扰沉默BmDHS基因对家蚕生长发育的影响研究初探

doi:10.7668/hbnxb.2011.02.003

华北农学报 ›› 2011, Vol. 26 ›› Issue (2): 13-16. doi: 10.7668/hbnxb.2011.02.003

所属专题： 生物技术；

RNA干扰沉默BmDHS基因对家蚕生长发育的影响研究初探

王更先^1,2, 司马杨虎¹, 张升祥³, 徐世清¹

1. 苏州大学基础医学与生物科学学院, 现代丝绸国家工程实验室, 江苏苏州, 215123;
2. 邯郸学院生物科学系, 河北邯郸, 056005;
3. 山东农业大学林学院, 山东泰安, 271018

收稿日期:2011-01-15 出版日期:2011-04-28
通讯作者: 徐世清(1963-),男,江苏人,教授,博士生导师,主要从事基因工程研究工作.
作者简介:王更先(1981-),男,山东人,博士,主要从事基因工程与生物信息学教学与研究.
基金资助:
国家重点基础研究发展计划"973"项目(2005CB121005)

Preliminary Study on the Effects of BmDHS Gene Silencing by RNAi on Growth and Development of the Silkworm, Bombyx mori

WANG Geng-xian^1,2, SIMA Yang-hu¹, ZHANG Sheng-xiang³, XU Shi-qing¹

1. State Key Laboratory of Modern Silk, Department of Applied Biology, Medical College, Soochow University, Suzhou 215153, China;
2. Department of Biological Science, Handan College, Handan 056005, China;
3. College of Forestry, Shandong Agricultural University, Taian 271018, China

Received:2011-01-15 Published:2011-04-28

摘要/Abstract

摘要： 用家蚕龄起蚕为材料,以桑抗冻蛋白基因dsRNA和ddH₂O为对照,利用微量注射RNA干扰技术将家蚕脱氧羟腐胺赖氨酸合酶基因BmDHS的dsRNA注入幼虫体内,抑制家蚕BmDHS基因表达,获得BmDHS基因沉默表型.通过RT-PCR方法检测沉默家蚕体内BmnDHS基因mRNA水平平均下降了约25%,BmDHS靶基因BmelF5A基因mRNA水平平均下降了约4.8%.注射后10 d平均体重增加了42.9%,在上簇后5d蚕茧的重量平均值增加了35.4%.初步证明BmDHS基因在家蚕生长发育中起重要作用.

关键词: 家蚕, BmDHS, 生长发育, RNA干扰

Abstract: By using mulberry antifreeze protein gene'dsRNA and autoclaved distilled water as control based on microinjection RNA interference technology, we have prelin inarily got BmDHS gene silence phenotype by injecting 4th larval instar. Compared with control group, in the silent silk worm, the average level of BmDHS mRNA was reduce almost 25%; the average level of BmelF5A mRNA was reduced about 4.8%;the average larval weight increased by 42.9%.10 days after injection; the average cocoons weght increased by 35.4% at the fifth day after mounting. It prelim inarily proofed that BmDHS gene plays an inportant role in silk worm's growth and development.

Key words: Bambyx mori, BmDHS, Growth and development, RNAi

中图分类号:

S881.2
S186

王更先, 司马杨虎, 张升祥, 徐世清. RNA干扰沉默BmDHS基因对家蚕生长发育的影响研究初探[J]. 华北农学报, 2011, 26(2): 13-16. doi: 10.7668/hbnxb.2011.02.003.

WANG Geng-xian, SIMA Yang-hu, ZHANG Sheng-xiang, XU Shi-qing. Preliminary Study on the Effects of BmDHS Gene Silencing by RNAi on Growth and Development of the Silkworm, Bombyx mori[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 13-16. doi: 10.7668/hbnxb.2011.02.003.

http://www.hbnxb.net/CN/Y2011/V26/I2/13

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RNA干扰沉默BmDHS基因对家蚕生长发育的影响研究初探

Preliminary Study on the Effects of BmDHS Gene Silencing by RNAi on Growth and Development of the Silkworm, Bombyx mori

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RNA干扰沉默BmDHS基因对家蚕生长发育的影响研究初探

Preliminary Study on the Effects of BmDHS Gene Silencing by RNAi on Growth and Development of the Silkworm, Bombyx mori

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