小麦生长、成熟和产量负调控基因<i>TaJIP2</i>的克隆及功能分析

doi:10.7668/hbnxb.20192370

华北农学报 ›› 2021, Vol. 36 ›› Issue (6): 35-44. doi: 10.7668/hbnxb.20192370

所属专题： 小麦；生物技术；

• 作物遗传育种·种质资源·生物技术 • 上一篇下一篇

小麦生长、成熟和产量负调控基因TaJIP2的克隆及功能分析

王晓桠¹, 卜瑞方^1,2, 胡海燕¹, 龙强¹, 孙连轩¹, 齐璐¹, 刘峥¹, 李逍瑶^1,2, 李成伟^1,3

1. 河南科技学院生命科技学院, 河南省粮食作物基因组编辑工程技术研究中心, 河南省植物遗传改良与土壤修复国际联合重点实验室, 河南新乡 453003;
2. 河南科技学院园艺园林学院, 河南新乡 453003;
3. 河南工业大学生物工程学院, 河南郑州 450001

收稿日期:2021-07-27 出版日期:2021-12-28
通讯作者: 李成伟(1972-),男,河南商丘人,教授,博士,主要从事植病互作和生物反应器研究。
作者简介:王晓桠(1996-),女,河南登封人,硕士,主要从事作物遗传育种研究。
基金资助:
国家自然科学基金面上项目（31872129）；国家自然科学基金青年基金项目（31902031）；中国博士后科学基金面上项目（2018M630821）；河南省博士后科研项目（001801020）

Cloning and Function Analysis of TaJIP2 Gene Negatively Regulating Growth, Maturity and Yield in Triticum aestivum L.

WANG Xiaoya¹, BU Ruifang^1,2, HU Haiyan¹, LONG Qiang¹, SUN Lianxuan¹, QI Lu¹, LIU Zheng¹, LI Xiaoyao^1,2, LI Chengwei^1,3

1. College of Life Science and Technology, Henan Engineering Research Center of Crop Genome Editing, Henan International Joint Laboratory of Plant Genetic Improvement and Soil Remediation, Henan Institute of Science and Technology, Xinxiang 453003, China;
2. College of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China;
3. College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China

Received:2021-07-27 Published:2021-12-28

摘要/Abstract

摘要： 为探究茉莉酸（JA）对小麦光合作用效率、小麦生长、成熟和产量的调控作用。试验采用同源克隆的方法，从小麦百农207中扩增到了一个小麦茉莉酸诱导蛋白基因TaJIP2，对TaJIP2进行组织差异表达分析，并构建TaJIP2基因RNA干扰（RNAi）载体后对小麦进行遗传转化及功能分析。结果表明，TaJIP2基因在各个部位均有表达，其中在颖壳中表达量最高；沉默TaJIP2基因显著提高JA生物合成关键酶丙二烯氧化物环化酶（AOC）和12-氧-植物二烯酸还原酶（OPR）活性和JA含量，其中根系中JA含量差异最大。沉默TaJIP2基因显著促进小麦根系和地上部分生长，其中根系生长状况差异更显著；根尖细胞超微结构观察结果显示，沉默TaJIP2基因小麦植株根尖细胞结构完整，细胞内各细胞器种类和数量丰富，细胞活性强，从而促进了根系生长。此外，沉默TaJIP2基因显著提高了小麦叶片光合作用效率，且抽穗时间提前，显著增加了小麦籽粒的粒长和粒宽及单株的穗数、穗粒数、千粒质量和单株产量。以上结果表明，RNAi诱导的TaJIP2基因沉默显著促进小麦植株生长、提前成熟和高产，表明TaJIP2是一个小麦生长、成熟和产量的关键负调控基因。

关键词: 小麦, 小麦茉莉酸诱导蛋白基因2(TaJIP2), RNA干扰, 促生长, 早熟, 高产

Abstract: To explore the regulation mechanism of Jasmonic acid(JA) on growth,maturation and yield in wheat,the gene TaJIP2 was cloned from wheat Bainong 207 by homologous cloning method.Analysis of tissue differential expression showed that TaJIP2 gene was expressed in all tissues and the expression level was the highest in wheat hull.The TaJIP2 gene RNA interference(RNAi) vector was constructed to conduct genetic transformation and functional analysis in wheat.The results showed that silencing TaJIP2 gene increased the key enzymes activities of jasmonic acid biosynthesis,allene oxide cyclase(AOC) and 12-oxo-phytodienoic acid reductase(OPR),and JA content,and the difference in JA content was mainly reflected in the roots.At the same time,it significantly promoted the growth of roots and above ground parts in wheat,and the difference was also mainly in root growth.In addition,observation of the ultrastructure of root tip cells showed that the root tip cells of TaJIP2 gene silenced plants were complete in structure,rich in variety and number of organelles,and strong in cell activity,thus promoting root growth.Silencing TaJIP2 significantly improved the photosynthetic efficiency of wheat leaves,made the heading time earlier,and significantly increased the grain length and width,spike number per plant,grain number per spike,1 000-grain weight and yield per plant.The results showed that RNAi-induced silencing of TaJIP2 significantly promoted the growth,early maturity and high yield of wheat,which indicated that TaJIP2 was a key negative regulator of wheat growth,maturity and yield.These findings provided genetic resources and research basis for promoting the photosynthetic efficiency,growth,early ripeness and high yield in wheat.

Key words: Wheat, Triticum aestivum jasmonate-induced protein 2(TaJIP2), RNA interference, Growth promotion, Early ripeness, High yield

中图分类号:

S512.01
Q78

王晓桠, 卜瑞方, 胡海燕, 龙强, 孙连轩, 齐璐, 刘峥, 李逍瑶, 李成伟. 小麦生长、成熟和产量负调控基因TaJIP2的克隆及功能分析[J]. 华北农学报, 2021, 36(6): 35-44. doi: 10.7668/hbnxb.20192370.

WANG Xiaoya, BU Ruifang, HU Haiyan, LONG Qiang, SUN Lianxuan, QI Lu, LIU Zheng, LI Xiaoyao, LI Chengwei. Cloning and Function Analysis of TaJIP2 Gene Negatively Regulating Growth, Maturity and Yield in Triticum aestivum L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 35-44. doi: 10.7668/hbnxb.20192370.

http://www.hbnxb.net/CN/Y2021/V36/I6/35

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小麦生长、成熟和产量负调控基因TaJIP2的克隆及功能分析

Cloning and Function Analysis of TaJIP2 Gene Negatively Regulating Growth, Maturity and Yield in Triticum aestivum L.

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小麦生长、成熟和产量负调控基因TaJIP2的克隆及功能分析

Cloning and Function Analysis of TaJIP2 Gene Negatively Regulating Growth, Maturity and Yield in Triticum aestivum L.

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