<i>PH-START1</i>调控拟南芥发育的功能分析

doi:10.7668/hbnxb.201751132

摘要/Abstract

摘要： 为明确PH-START1（At2g28320）调控拟南芥生长发育的功能，利用Real-time PCR技术对PH-START1在不同生长时期拟南芥的不同器官中的表达情况进行了分析，明确了PH-START1的时空表达特性；通过创制PH-START1功能获得和缺失的转基因拟南芥并分析其表型，明确了PH-START1在调控拟南芥生长发育过程中的生物学功能。Real-time PCR结果表明：PH-START1在拟南芥的根、叶、花、角果中都有表达。在幼苗期的根中表达量最多，在成苗期根中表达量逐渐减少；莲座叶中第6片莲座叶表达量最多，然后随着发育时间的延长逐渐减少；在花的发育过程中，随着花发育时间延长，PH-START1的表达量逐渐增多；授粉后3~5 d的角果中PH-START1的表达量最高，然后表达量逐渐降低，到授粉后9 d角果中PH-START1的表达量又略有增加。花中PH-START1在雄蕊中的表达量最高，其次是花瓣、萼片，在雌蕊中表达量最少。对PH-START1功能获得（过表达，OE）和缺失（T-DNA插入，ph-start1）的转基因拟南芥表型进行观察，分析PH-START1在调控拟南芥生长发育中的功能，发现过表达PH-START1拟南芥营养生长受到明显的抑制，地上部株型矮小、茎细弱、叶片明显变小，地下部根系发育也受到明显的抑制。这说明PH-STARTI在拟南芥生长发育中起负调控作用，为阐明和促控植物生长发育提供了理论依据。

关键词: PH-START1, 拟南芥, 生长发育, Real-time PCR

Abstract: In order to clarify the function of PH-START1 (At2g28320) in regulating the growth and development of Arabidopsis, the expression of PH-START1 in different organs of Arabidopsis during different growth periods was analyzed by Real-time PCR, and the space-time expression characteristics of PH-START1 was determined. The PH-START1 function-obtaining and function-missing transgenic Arabidopsis were created and their phenotypes were analyzed. The biological function of PH-START1 in regulating the growth and development of Arabidopsis was elucidated. The Real-time PCR results showed that the expression of PH-START1 gene presented in the roots, leaves, flowers and siliques, and the highest expression level of PH-START1 was found in roots at seedling stage. The highest expression of the gene was in the 6th rosette leaf, then gradually decreased as the development time extended. In the development period of flowers, the expression of PH-START1 increased gradually with the development time of flowers. The highest expression of PH-START1 in kernels presented in 3-5 d after pollination, and then gradually decreased, but the expression of PH-START1 increased slightly in 9 d kernels after pollination. The highest expression of PH-START1 was in stamens, followed by petals and sepals, and the least in pistil. In order to analyze the function of PH-START1 in regulating the growth and development of Arabidopsis, the phenotype of the gain-of-function (overexpression, OE) and deletion (T-DNA insertion, ph-start1) transgenic Arabidopsis of PH-START1 was observed. The vegetative growth of PH-START1 overexpressing Arabidopsis was significantly inhibited, presenting shorter plant, weaker stems, smaller leaves and less roots. This suggested that PH-STARTI had a negative regulatory role in the growth and development of Arabidopsis, which provided a theoretical basis for elucidating and promoting the plant growth and development.

Key words: PH-START1, Arabidopsis thaliana, Growth and development, Real-time PCR

中图分类号:

Q945.49

赵亚卓, 王鑫, 冯佳佳, 孙丹丹, 王凤茹, 董金皋. PH-START1调控拟南芥发育的功能分析[J]. 华北农学报, 2019, 34(2): 87-94. doi: 10.7668/hbnxb.201751132.

ZHAO Yazhuo, WANG Xin, FENG Jiajia, SUN Dandan, WANG Fengru, DONG Jingao. Functional Analysis of PH-START1 in Regulating Growth and Development of Arabidopsis thaliana[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(2): 87-94. doi: 10.7668/hbnxb.201751132.

http://www.hbnxb.net/CN/Y2019/V34/I2/87

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PH-START1调控拟南芥发育的功能分析

Functional Analysis of PH-START1 in Regulating Growth and Development of Arabidopsis thaliana

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