华北农学报 ›› 2004, Vol. 19 ›› Issue (1): 40-43. doi: 10.3321/j.issn:1000-7091.2004.01.012

所属专题: 小麦 盐碱胁迫

• 论文 • 上一篇    下一篇

小麦与多枝赖草耐盐纯合易位系的培育及GISH鉴定

魏景芳1, 秦君2, 王淳1, 李冬杰1, 孙敬三3   

  1. 1. 河北科技大学, 生命科学与工程学院, 河北, 石家庄, 050018;
    2. 河北省农林科学院, 河北, 石家庄, 050051;
    3. 中科院植物研究所, 北京, 100044
  • 收稿日期:2003-12-18 出版日期:2004-03-28
  • 作者简介:魏景芳(1957- ),男,河北黄骅人,教授,博士,主要从事细胞工程与小麦种质资源创新研究工作。
  • 基金资助:
    河北省自然科学基金项目(301443)

Development and GISH Identification of Salt-tolerant Translocation Lines Between Wheat and Leymus multicaulis

WEI Jing-fang1, QIN Jun2, WANG Chun1, LI Dong-jie1, SUN Jing-san3   

  1. 1. Hebei University of Science and Technology , Shijiazhuang050018, China;
    2. Hebei Academy of Agricultural and Forestry Sciences, Shjiazhuang050051, China;
    3. Institute of Botany, Chinese Academy of Sciences, Beijing100044, China
  • Received:2003-12-18 Published:2004-03-28

摘要: 以小麦与多枝赖草属间杂种花药培养获得的纯合后代纯系为材料,经过细胞学观察、田间选拔、抗性鉴定、综合农艺性状调查以及小区产量对比试验,从中初步筛选鉴定出具有外源耐盐性状、且农艺性状好的材料,再经分子标记(GISH)鉴定纯合易位系。表明花药培养可有效克服小麦远缘杂交后代的疯狂分离,并可促进染色体的结构变异,在短时间内获得大量具有丰富变异且可稳定遗传的后代,大大缩短了小麦远缘杂交导入外源基因的年限。

关键词: 小麦, 多枝赖草, 属间杂种, 花药培养, 易位系GISH

Abstract: Pure lines from antherulture of intergeneric hybrids between wheat and Leymus multacaulaswere used to develop pure translocation lines with salrtolerance and ideal agronomicharacteristics through cytlogical detection,field selection,resistance assessment,omprehensive observation of agricultural characteristics,yield trials,and molecular marks(GISH).

Key words: Wheat, Leymus mutacaulas, Intergeneric hybrids, Anther culture, Translocation lines, GISH

中图分类号: 

引用本文

魏景芳, 秦君, 王淳, 李冬杰, 孙敬三. 小麦与多枝赖草耐盐纯合易位系的培育及GISH鉴定[J]. 华北农学报, 2004, 19(1): 40-43. doi: 10.3321/j.issn:1000-7091.2004.01.012.

WEI Jing-fang, QIN Jun, WANG Chun, LI Dong-jie, SUN Jing-san. Development and GISH Identification of Salt-tolerant Translocation Lines Between Wheat and Leymus multicaulis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2004, 19(1): 40-43. doi: 10.3321/j.issn:1000-7091.2004.01.012.