摘要： 以土作为培养基支撑物，离体培养葡萄试管苗双节茎段，40天后即达到适于炼苗标准，成苗率95.8%.带坨移入营养钵，在不喷雾、不覆膜、空气湿度低至45%条件下，成活率达96.7%.成活的试管苗炼苗54天后移入大田，移栽成活率为100%，其中特级苗占87.5%，一级苗占12.5%.试验证明，土支撑培养带坨移栽技术是简化试管苗移栽程序、降低成本的有效措施。

关键词: 葡萄, 试管苗, 带坨移栽

Abstract: Stem sections with two nodes from in vitro cultured grape plantlets were cultured in soil supporting medium for 40 days, 95.8% of the stem sections produced new plantlets which subsequently underwent a four-week-long acclimatization. The ptantlets were then transplanted with lump into soil, and 96.7% of them survived under the conditions of no spraying of water,no plastic film covering, and a low relative air humidity of 45%. 54 days later, the survived plantlets were further transported into field, and in autumn they all formed young plants in which the super-grade plants and first-grade plants accounted for 87.5% and 12.5%respectively. The results showed that the techniques described above can effectively simplify the transplant procedure and reduce the cost of the plantlets derived from in vitro culture.

Key words: Grape, Plantlet in vitro, Transplanting with lump

中图分类号: 

• S663.104.4